ERK1 ELISA Kit

Catalog No. ABIN2533189

This Rat ERK1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat ERK1.

Quick Overview for ERK1 ELISA Kit (ABIN2533189)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 125 pg/mL - 8000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details ERK1 ELISA Kit

Minimum Detection Limit: 125 pg/mL

Purpose: Rat Mitogen-Activated Protein Kinase 3 / ERK1 (MAPK3) ELISA Kit

Analytical Method: Quantitative

Sensitivity: 75 pg/mL

Components: The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

• Pre-coated 96-Well Microplate
• Standard
• Standard Diluent Buffer
• Wash Buffer
• Detection Reagent A
• Detection Reagent B
• Diluent A
• Diluent B
• TMB Substrate
• Stop Solution
• Plate Sealer

Material not included:

• 37 °C incubator
• Multi and single channel pipettes and sterile pipette tips
• Squirt bottle or automated microplate washer
• 1.5 mL tubes
• Distilled water
• Absorbent filter papers
• 100 mL and 1 liter graduated cylinders
• Microplate reader (wavelength: 450 nm)
• ELISA Shaker

Application Details

Application Notes: Optimal dilutions/concentrations should be determined by the end user.

Comment:

The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

Plate: Pre-coated

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Mitogen-Activated Protein Kinase 3 / ERK1 (MAPK3) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Mitogen-Activated Protein Kinase 3 / ERK1 (MAPK3) were tested on 3 different plates, 8 replicates in each plate.

CV (%) = (Standard Deviation / mean) x 100

Intra-Assay: CV<10%

Inter-Assay: CV<10%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

Expiry Date: 6 months

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: Mitogen-Activated Protein Kinase 3

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins