MMAE Antibody Drug Conjugate (ADC) ELISA Kit
Reactivity: Human
Colorimetric
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- Target
- MMAE Antibody Drug Conjugate (ADC)
- Reactivity
- Human
- Detection Method
- Colorimetric
- Application
- ELISA
- Sensitivity
- 0.0101 μg/ml
- Characteristics
- Human MMAE Antibody Drug Conjugate (ADC) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for use in the quantitative determination of antibody-MMAE-conjugate level in test sample. It is useful for pre-clinical and clinical pharmacology study of MMAE Antibody Drug Conjugate (ADC).
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Sample Volume
- 100 μL
- Assay Time
- 2.5 h
- Protocol
- Assay calibrators (antibody MMAE conjugate) and test serum samples are added directly to wells of a microtiter plate that is coated with specific anti-MMAE antibody. Subsequently, a horseradish peroxidase (HRP) conjugated MMAE is added to each well. During the incubation period, the antibody MMAE conjugate competes with the HRP conjugated MMAE for the limited binding sites of anti-MMAE antibody. An immune complex of well coated "anti-MMAE antibody - HRP conjugated MMAE" is formed. The unbound antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction, which is terminated with an acidic reagent (i.e. ELISA stop solution). The absorbance is then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is inversely proportional to the amount of antibody-MMAE conjugate in the test sample. A calibration curve is generated by plotting the absorbance versus the respective antibody-MMAE conjugate concentration for each calibrator on a 4-parameter or log-logit curve fitting. The concentration of antibody-MMAE conjugate in test samples is determined directly from this calibration curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
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- Target
- MMAE Antibody Drug Conjugate (ADC)
- Target Type
- Antibody
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