Anti-Nuclear Antibody (ANA) ELISA Kit

Catalog No. ABIN2648251

Product Details

Target: Anti-Nuclear Antibody (ANA)

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Purpose: Enzyme immunoassay for the determination of IgG antibodies to nuclear and cytoplasmic antigensin human serum and plasma

Brand: ANAscreen

Sample Type: Serum, Plasma

Analytical Method: Semi-Quantitative

Specificity: Frequency distribution of ANA in ANAscreen: 97unselected human sera from healthy donors weretested. All sera were found negative. Thiscorresponds to a diagnostic specificity of 100%.

Characteristics: ANAscreenallows the detection of total autoantibodiesto nuclear and cytoplasmic antigens in one sample as asummary parameter in the diagnosis of systemicautoimmunedisorders.Antigeniccombinationofcomplete HeLa nuclei with recombinant proteins andpurified native antigens guarantees a maximum ofsensitivy and specificity for the ANA detection.

Components: Microtiter plate
Concentrated wash buffer
Sample diluent
Conjugate
Substrate
Stop solution
Positive Control
Cut-off Control
Negative Control

Material not included: micropipettes
multi-channel pipette or multi-pipettetrough for multi-channel pipette
8-channel wash comb with vacuum pump and wastebottle or microplate washer
distilled or de-ionized water
glassware

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol: The ANAscreen ELISA Assay Kit is an enzymeimmunoassay for the semi-quantitative determination of IgG antibodies to nuclear and cytoplasmic antigens. Antibodies of the controls and diluted patient samples react with nuclear and cytoplasmic antigens immobilized on the solid phase of microtiter plates. Use of complete HeLa nuclei enriched with recombinant and native antigens guarantees the specific binding of autoimmune antibodies of the specimen under investigation. Following an incubation period of 60 min at room temperature (RT,18...25°C),unbound sample components are removed by a wash step. The bound IgG antibodies react specifically with anti-human-IgG conjugated to horseradish peroxidase (HRP). Within the incubation period of 30 min at RT, excessive conjugate is separated from the solid-phase immunecomplexes by the following wash step. HRP converts the colorless substrate solution of 3,3’,5,5’-tetramethylbenzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispensing an acidic solution into the wells after 15 min at room temperature turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specific antibodies bound. Patient ratios are calculated by dividing the respective OD of the specimen with the calculated cut-off OD.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Upon receipt, all components of the ANAscreen have tobe kept at 2 - 8 °C, preferably in the original kit box.

Target Details

Target: Anti-Nuclear Antibody (ANA)

Alternative Name: ANA (Antinuclear Antibody) (ANA Products)

Synonyms: ANA ELISA Kit, TOB5 ELISA Kit, TOB55 ELISA Kit, TOFA ELISA Kit, BTG anti-proliferation factor 3 ELISA Kit, BTG3 ELISA Kit

Target Type: Antibody, Antibody

Background: Systemic autoimmune diseases such as systemic lupuserythematosus,scleroderma,rheumatoidarthritis,Sjögren’s syndrome, dermatomyositis, mixed connectivetissue disease are characterized by the appearance of avariety of autoantibodies directed against componentsof the cell nucleus.Although significance and pathological relevance ofsome auto-antibodies are not completely revealed yet,the detection of auto-antibodies is widely establishedand plays an important role in the diagnosis of systemicautoimmune diseases.