Endothelin Big ELISA Kit

Catalog No. ABIN2648509

Human Endothelin Big ELISA Kit, Colorimetric assay for quantification of Human Endothelin Big.

Quick Overview for Endothelin Big ELISA Kit (ABIN2648509)

Target: Endothelin Big

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum, Plasma, Plasma (EDTA)

Product Details Endothelin Big ELISA Kit

Sensitivity: 0.02 pmol/L

Characteristics: Big Endothelin-1 (BigET) is a peptide of 38 amino acids and is the precursor of Endothelin-1 (ET), represented by amino acids 1-21 (http://www.uniprot.org/uniprot/P05305). ET is a potent vasoconstrictor and is produced by vascular endothelial cells. Accordingly it has a wide tissue distribution (http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Hs.511899). The cleavage of BigET by Endothelin Converting Enzyme (ECE) leads to ET and to a C-terminal fragment. Both BigET and ET are strong independent predictors of survival in patients with congestive heart failure, and identify a population with a very high risk mortality. The half-life of ET (1-21) in plasma is less than one minute, whereas clearance of BigET is much slower. BigET can therefore be determined more easily.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 50 μL

Assay Time: 5.5 h

Plate: Pre-coated

Protocol: Human Big Endothelin-1 ELISA Assay Kit in serum & plasma. Big Endothelin-1 ELISA Assay Kit is manufactured by Biomedica Gruppe (www.bmgrp.com) and is for research use only, not to be used in diagnostic procedures.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for Endothelin Big

Target: Endothelin Big

Alternative Name: Big Endothelin