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CD14 ELISA Kit

Human CD14 ELISA Kit Colorimetric assay for quantification of Human CD14.

Catalog No. ABIN2648533

Quick Overview for CD14 ELISA Kit (ABIN2648533)

Target

See all CD14 ELISA Kits

CD14

Reactivity

8
6
3
2
2
2
2
1
1
1
1
1
1

Human

Detection Method

Colorimetric

Application

ELISA

Sample Type

Serum, Plasma, Cell Culture Cells

Discover our top product CD14 ELISA Kit

Purpose

Human CD14 ELISA Assay kit is to be used for the in-vitro quantitative determination of CD14 in serum, plasma, or cell lysates. The assay will recognize both natural and recombinant CD14.

Analytical Method

Quantitative

Sensitivity

6 ng/mL

Characteristics

Human CD14 ELISA Assay kit is for Research Use Only.
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CD14 Reactivity: Pig Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum

Application Notes

Optimal working dilution should be determined by the investigator.

Sample Volume

100 μL

Assay Time

3 - 4 h

Plate

Pre-coated

Protocol

The Human CD14 ELISA Assay kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for CD14 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD14 concentrations and unknowns are pipetted into these wells. During the first incubation, the CD14 antigen, standards and zeros are bound this is followed by a separate incubation where the biotinylated monoclonal antibody specific for CD14 is added.

Restrictions

For Research Use only
Storage

4 °C
Target See all CD14 ELISA Kits

CD14

Alternative Name

CD14

Pathways

TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades

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