CD25 ELISA Kit
Quick Overview for CD25 ELISA Kit (ABIN2648538)
Target
See all CD25 (IL2RA) ELISA KitsReactivity
Detection Method
Method Type
Application
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Purpose
- sCD25 ELISA kit is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of sCD25 in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant human sCD25.
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Analytical Method
- Qualitative and Quantitative
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Characteristics
- Human sCD25 ELISA Assay kit is for Research Use Only.
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Interleukin 2 Receptor, alpha (IL2RA) ELISA Kit
IL2RA Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA Reactivity: Mouse Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA Reactivity: Cow Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA Reactivity: Mouse AA 22-236 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA Reactivity: Human AA 22-213 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA Reactivity: Human Soluble Colorimetric Sandwich ELISA 78.13-5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 2 Receptor, alpha (IL2RA) ELISA KitIL2RA Reactivity: Mouse Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Pre-coated
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Protocol
- A capture Antibody highly specific for CD25 has been coated to the wells of the microtitre strip plate provided during manufacture. Binding of CD25 samples and known standards to the capture antibodies and subsequent binding of the biotinylated anti-CD25 secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of CD25 present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of CD25 in any sample tested.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
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Alternative Name
- CD25 / IL-2R
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Pathways
- JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
Target See all CD25 (IL2RA) ELISA Kits
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