CD25 ELISA Kit

Catalog No. ABIN6719931

Product Details CD25 ELISA Kit

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: CD122, CD122 antigen, CD132, CD132 antigen, CD25, CD25 antigen, Cytokine receptor common gamma chain, Gamma C, High affinity IL 2 receptor subunit beta, IL 2 RA, IL 2 receptor, IL 2 receptor alpha subunit, IL 2R gamma chain, IL2R, IL2RA, IL2RB, IL2RG, IMD4, interleukin 2 receptor alpha, Interleukin 2 receptor alpha chain, interleukin 2 receptor beta, Interleukin 2 receptor beta chain, interleukin 2 receptor gamma, Interleukin 2 receptor gamma chain, p55, p64, P70 75, p75, SCIDX, SCIDX1, TAC antigen, TCGFR, XSCID

Background: CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.

Gene Name: IL2RA

Production: Natural and recombinant mouse CD25. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: E22-K236

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL mouse CD25 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. We recommend that each mouse CD25 standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 78pg/mL-5000pg/mL

Other Performance Data: Expiration Dates: 4° C - 2/8/18 -20° C - 8/9/18

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25 - IL-2sR alpha (IL2RA Products)

Synonyms: CD25 ELISA Kit, IDDM10 ELISA Kit, IL2R ELISA Kit, TCGFR ELISA Kit, IL2RAC ELISA Kit, IL-2RA ELISA Kit, Il2r ELISA Kit, Ly-43 ELISA Kit, interleukin 2 receptor subunit alpha ELISA Kit, interleukin-2 receptor subunit alpha-like ELISA Kit, interleukin 2 receptor, alpha chain ELISA Kit, IL2RA ELISA Kit, Il2ra ELISA Kit, LOC100222957 ELISA Kit

Gene ID: 16184

NCBI Accession: NP_032393

UniProt: P01590

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation