IL-6 Receptor ELISA Kit

Catalog No. ABIN2648539

Human IL-6 Receptor ELISA Kit Colorimetric assay for quantification of Human IL-6 Receptor.

Quick Overview for IL-6 Receptor ELISA Kit (ABIN2648539)

Target: IL-6 Receptor (IL6R) (Interleukin 6 Receptor (IL6R))

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Product Details IL-6 Receptor ELISA Kit

Purpose: Human IL-6R/CD126 ELISA is to be used for the in-vitro quantitative determination of soluble IL-6 Receptor (IL-6R) (CD126) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human IL-6R.

Analytical Method: Quantitative

Characteristics: Human IL-6R/CD126 ELISA Assay kit is for Research Use Only.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol: The IL-6R Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for IL-6R has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6R concentrations and unknowns are pipetted into these wells. During the first incubation, the IL-6R antigen and a biotinylated monoclonal antibody specific for IL-6R are simultaneously incubated. After washing, the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-6R present in the samples.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for IL-6 Receptor

Target: IL-6 Receptor (IL6R) (Interleukin 6 Receptor (IL6R))

Alternative Name: CD126 / Gp80

Pathways: JAK-STAT Signaling, Autophagy, Growth Factor Binding, Cancer Immune Checkpoints