CD86 ELISA Kit
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- Target See all CD86 ELISA Kits
- CD86
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Reactivity
- Human
- Detection Method
- Colorimetric
- Application
- ELISA
- Purpose
- Human CD86 ELISA is to be used for the in-vitro quantitative determination CD86 in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human CD86.
- Sample Type
- Serum, Plasma, Cell Culture Cells
- Analytical Method
- Quantitative
- Sensitivity
- 0.6 U/ml
- Characteristics
- Human CD86 ELISA Assay kit is for Research Use Only.
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- Discover our top product CD86 ELISA Kit
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Sample Volume
- 100 μL
- Assay Time
- 2 - 3 h
- Plate
- Pre-coated
- Protocol
- The CD86 Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for CD86 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD86 concentrations and unknowns are pipetted into these wells. During the first incubation, the CD86 antigen and a biotinylated monoclonal antibody specific for CD86 are simultaneously incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD86 present in the samples.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
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- Target See all CD86 ELISA Kits
- CD86
- Alternative Name
- CD86 (CD86 Products)
- Synonyms
- B7-2 ELISA Kit, B7.2 ELISA Kit, B70 ELISA Kit, CD28LG2 ELISA Kit, LAB72 ELISA Kit, B7 ELISA Kit, CLS1 ELISA Kit, Cd28l2 ELISA Kit, ETC-1 ELISA Kit, Ly-58 ELISA Kit, Ly58 ELISA Kit, MB7 ELISA Kit, MB7-2 ELISA Kit, TS/A-2 ELISA Kit, CD86 molecule ELISA Kit, CD86 antigen ELISA Kit, integrin subunit alpha 2b ELISA Kit, CD86 ELISA Kit, Cd86 ELISA Kit, Itga2b ELISA Kit
- Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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