Cortisol ELISA Kit
Quick Overview for Cortisol ELISA Kit (ABIN2648588)
Target
See all Cortisol ELISA KitsReactivity
Detection Method
Application
Sample Type
-
-
Sensitivity
- 2.95 ng/ml
-
Characteristics
- Cortisol is a steroid hormone released from the adrenal cortex in response to an hormone called ACTH (produced by the pituitary gland), it is involved in the response to stress, it increases blood pressure, blood sugar levels, may cause infertility in women, and suppresses the immune system. Cortisol acts through specific intracellular receptors and has effects in numerous physiologic systems, including immune function, glucose-counter regulation, vascular tone, substrate utilization and bone metabolism. Cortisol is excreted primarily in urine in an unbound (free) form. Cortisol is bound, in plasma, from corticosteroid-binding globulin (CBG, transcotin), with high affinity, and from albumin. Only free cortisol is available to most receptors. These normal endogenous functions are the basis for the physiological consequences of chronic stress - prolonged cortisol secretion causes muscle wastage, hyperglycaemia, and suppresses immune / inflammatory responses. The same consequences arise from long-term use of glucocorticoid drugs.
-
-
Cortisol ELISA Kit
Reactivity: Various Species Colorimetric Sandwich ELISA Fecal, Hair, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Tissue Culture Medium, Urine
Cortisol ELISA KitReactivity: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Plasma, Serum, Urine
Cortisol ELISA KitReactivity: Various Species Colorimetric Sandwich ELISA 100 pg/mL - 3200 pg/mL Fecal, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Tissue Culture Medium, Urine
Cortisol ELISA KitReactivity: Various Species Colorimetric Competition ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Cortisol ELISA KitReactivity: Mouse Colorimetric Competition ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Cortisol ELISA KitReactivity: Rabbit Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Plasma, Serum, Urine
Cortisol ELISA KitSmall Sample Reactivity: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Plasma, Serum, Urine
Cortisol ELISA KitHigh Sensitivity Reactivity: Various Species Colorimetric Competition ELISA 2.47 ng/mL - 200 ng/mL Plasma, Serum, Urine
Cortisol ELISA KitReactivity: Human Colorimetric Competition ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Cortisol ELISA KitReactivity: Monkey Colorimetric Competition ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
-
-
Application Notes
- Optimal working dilution should be determined by the investigator.
-
Sample Volume
- 10 μL
-
Assay Time
- 1.5 h
-
Protocol
- The Cortisol (antigen) in the sample competes with the antigenic Cortisol conjugated with horseradish peroxidase (HRP) for binding to the limited number of antibodies anti Cortisol coated on the microplate (solid phase). After incubation, the bound/free separation is performed by a simple solid-phase washing. Then, the enzyme HRP in the bound-fraction reacts with the Substrate (H2O2) and the TMB Substrate and develops a blue color that changes into yellow when the Stop Solution (H2SO4) is added. The color intensity is inversely proportional to the Cortisol concentration of in the sample.Cortisol concentration in the sample is calculated through a calibration curve.
-
Restrictions
- For Research Use only
-
-
-
Storage
- 4 °C
-
-
- Cortisol
-
Target Type
- Hormone
Target See all Cortisol ELISA Kits
-
