Proteinase 3 Anti-Neutrophil Cytoplasmic Antibody (PR3 cANCA) ELISA Kit

Catalog No. ABIN2648603

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Proteinase 3 Anti-Neutrophil Cytoplasmic Antibody.

Quick Overview for Proteinase 3 Anti-Neutrophil Cytoplasmic Antibody (PR3 cANCA) ELISA Kit (ABIN2648603)

Target: Proteinase 3 Anti-Neutrophil Cytoplasmic Antibody (PR3 cANCA)

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum, Plasma

Product Details

Sensitivity: 0.13 AU/mL

Characteristics: Anti-neutrophilic-cytoplasm antibodies (ANCA) represents a group of autoantibodies directed towards the cytoplasmatic components of the neutrophilic granulocytes and monocytes. The classical methods for the determination of ANCA are the immunofluorescent methods. With these indirect immunofluorescence techniques two main patterns are recognized, a cytoplasmatic (c-ANCA) and a perinuclear (p-ANCA) type.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 10 μL

Assay Time: 1.5 h

Protocol: Anti PR3 (c-ANCA) ELISA Assay Kit is a test is based on the binding of the antibodies in the sample with human neutrophil proteinase 3 coated into the microplates. In the first step the antibodies in calibrators, controls or prediluted patient samples bind to the inner surface of the wells. After a 60 minutes incubation the microplate is washed with wash buffer for removing non-reactive serum components. In the second step an anti-human-IgG horseradish peroxidase conjugated solution recognizes the IgG class antibodies bound to the immobilized antigens. After a 30 minutes incubation any excess enzyme conjugate which is not specifically bound is washed away with the wash buffer.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details

Target: Proteinase 3 Anti-Neutrophil Cytoplasmic Antibody (PR3 cANCA)

Alternative Name: ANTI PR-3 (C-ANCA)

Target Type: Antibody