ACA-IgG ELISA Kit

Catalog No. ABIN2648608

Human ACA-IgG ELISA Kit, Colorimetric assay for quantification of Human ACA-IgG.

Quick Overview for ACA-IgG ELISA Kit (ABIN2648608)

Target: ACA-IgG (Anti-Cardiolipin Antibody IgG (ACA-IgG))

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum, Plasma

Product Details ACA-IgG ELISA Kit

Sensitivity: 0.08 μgEq/m

Characteristics: The first study on the anti-phospholipid antibodies began in 1906 when Wasserman introduced a serological test for syphilis. In 1942 it was discovered that the active component is a phospholipid called Cardiolipina. In the 1950's it was observed that a large number of people appeared to be positive for syphilis tests but did not show any evidence of disease. Initially the phenomenon was classified as a series of false positive syphilis tests, before a more accurate analysis revealed, for this group of patients, a high prevalence of autoimmune disorders including SLE and Sjögrens syndrome.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 10 μL

Assay Time: 2 h

Protocol: Anti Cardiolipin IgG ELISA Assay Kit is based on the binding of antibodies on human serum or plasma directed against the antigenic complex between Cardiolipina and β2-Glycoprotein, this complex is coated on the microplate. In the first step the antibodies present in calibrators, controls or prediluted samples bind to this coated antigen. After a 60 minute incubation the microplate is washed with a wash buffer to remove the non-reactive serum or plasma components. Then an anti-human IgM horseradish peroxidase conjugated solution recognizes IgM class antibodies bound to the immobilized antigens. After a 60 minute incubation any excess of enzyme conjugate, which is not specifically bound, is washed away with the wash buffer. Finally, achromogenic substrate solution containing TMB is dispensed into the wells. After a 15 minute incubation the color development is stopped by adding the stop solution. The solution turns yellow at this point. The level of color is directly proportional to the concentration of IgG antibodies present in the original sample. The concentration of IgG antibodies in the sample is calculated through a calibration curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for ACA-IgG

Target: ACA-IgG (Anti-Cardiolipin Antibody IgG (ACA-IgG))

Alternative Name: ANTI CARDIOLIPIN IgG

Target Type: Antibody