Anti-Cardiolipin Antibody ELISA Kit

Catalog No. ABIN2648616

The Human Anti-Cardiolipin Antibody ELISA Kit (ABIN2648616) is a Colorimetric ELISA Kit designed to quantify Human Anti-Cardiolipin Antibody.

Quick Overview for Anti-Cardiolipin Antibody ELISA Kit (ABIN2648616)

Target: Anti-Cardiolipin Antibody (ACA)

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum, Plasma

Product Details Anti-Cardiolipin Antibody ELISA Kit

Sensitivity: 0.08 AU/ml

Characteristics: Anti Cardiolipin Screen ELISA Assay Kit allows to determine the unknown concentration of auto-antibodies directed against the Cardiolipin-β2-glycoprotein complex through two different calibration curve (one specific for IgG test, one specific for IgM test), two different conjugates linked to horseradish peroxidase (one specific for IgG test, the other specific for IgM test) and one microplate only. The principle of the method and the procedure are the same in both the tests. Use reagents for IgG or reagents for IgM depending on the isotype which is under investigation.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 10 μL

Assay Time: 2.5 h

Protocol: The first study on the anti-phospholipid antibodies began in 1906 when Wasserman introduced a serological test for syphilis. In 1942 it was discovered that the active component is a phospholipid called Cardiolipin. In the 1950's it was observed that a large number of people appeared to be positive for syphilis tests but did not show any evidence of disease. Initially the phenomenon was classified as a series of false positive syphilis tests, before a more accurate analysis revealed, for this group of patients, a high prevalence of autoimmune disorders including SLE and Sjögrens syndrome. The term lupus anticoagulant (LA), used for the first time in 1972, derives from experimental observations in which an increased risk of thrombosis was observed, paradoxically, with the presence of some anticoagulants factors, the term LA is not totally correct, in fact the disease is present more frequently in patients without lupus and it is associated with thrombosis rather than to abnormal bleeding.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for Anti-Cardiolipin Antibody

Target: Anti-Cardiolipin Antibody (ACA)

Alternative Name: ANTI CARDIOLIPIN

Target Type: Antibody