Insulin Autoantibody ELISA Kit

Catalog No. ABIN2648679

The Human Insulin Autoantibody ELISA Kit (ABIN2648679) is a Colorimetric ELISA Kit designed to quantify Human Insulin Autoantibody.

Quick Overview for Insulin Autoantibody ELISA Kit (ABIN2648679)

Target: Insulin Autoantibody

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum

Product Details

Sensitivity: 0.08 U/mL

Characteristics: Type 1 diabetes, also known as insulin-dependent diabetes mellitus (IDDM), results from a chronic autoimmune destruction of the insulin-secreting pancreatic beta cells, probably initiated by exposure of genetically susceptible host to an environmental agent. Autoimmune destruction of beta cells is thought to be completely asymptomatic until 80-90 % of the cells are lost. This process may take years to complete and may occur at any time in all ages.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 5 μL

Assay Time: 1.5 h

Plate: Pre-coated

Protocol: In the first step Insulin AAb from the diluted sample bind to human recombinant insulin coated on the microtiter plate. After an incubation of 60 minutes at 37 °C unbound components are removed by washing. In a next step bound antibodies reacts with the added anti-human-IgG horseradish peroxidase (HRP) complex. Excessive conjugate is removed after 15 minutes at 37 °C by another washing step. HRP converts the colorless substrate TMB added into a blue product. The enzyme reaction is stopped by adding an acid solution after 15 minutes at 37 °C. The absorbance of the resulting yellow product is measured at 450 / 620 nm within 30 minutes. The obtained OD is direct proportional to the amount of bound antibodies.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details

Target: Insulin Autoantibody

Alternative Name: IAA (Insulin Autoantibodies)

Target Type: Antibody