IL-8 ELISA Kit
Quick Overview for IL-8 ELISA Kit (ABIN2648705)
Target
See all IL-8 (IL8) ELISA KitsReactivity
Detection Method
Application
Sample Type
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Purpose
- Human Interleukin 8 (IL-8) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 8 (IL-8) concentrations in cell culture supernates, serum, and plasma.
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Analytical Method
- Quantitative
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Sensitivity
- 4 pg/ml
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Characteristics
- Human Interleukin 8 (IL-8) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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Interleukin 8 (IL8) ELISA Kit
IL8 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 8 (IL8) ELISA KitIL8 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 8 (IL8) ELISA KitIL8 Reactivity: Dog Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 8 (IL8) ELISA KitIL8 Reactivity: Human AA 28-99 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 8 (IL8) ELISA KitIL8 Reactivity: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 8 (IL8) ELISA KitIL8 Reactivity: Chicken Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 8 (IL8) ELISA KitIL8 Reactivity: Rabbit Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 8 (IL8) ELISA KitIL8 Reactivity: Horse Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 8 (IL8) ELISA KitIL8 Reactivity: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Sample Volume
- 100 μL
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Assay Time
- 3.5 h
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Plate
- Pre-coated
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Protocol
- This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-8 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-8 is added to the wells and binds to the combination of capture antibody-IL-8 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-8 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-8 standard dilutions and IL-8 sample concentration determined.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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- IL-8 (IL8) (Interleukin 8 (IL8))
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Alternative Name
- IL-8
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Pathways
- TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy
Target See all IL-8 (IL8) ELISA Kits
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