Vitamin B2 Whole Blood Microtiter Plate Assay
BCA
Reactivity: Chemical
Blood
Catalog No. ABIN2648738
$2,038.08
Plus shipping costs $50.00
96 tests
Shipping to:
United States
Delivery in 6 to 7 Business Days
Quick Overview for Vitamin B2 Whole Blood Microtiter Plate Assay (ABIN2648738)
Target
See all Vitamin B2 Kits
Vitamin B2
(Vitamin B2 (Riboflavin))
Reactivity
Chemical
Application
Biochemical Assay (BCA)
Sample Type
Blood
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Purpose
- Vitamin B2 is a microtiter plate test kit based on a microbiological assay which measures the total Vitamin B2 content in EDTA whole blood.The test kit contains all required reagents, e.g.standard,medium and microtiter plate coated with a specific microorganism, sufficient for 96 determinations including standard curves. An ELISA reader is required for evaluation of the Vitamin B2 content. For use in human and veterinary medicine and in research.
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Sensitivity
- 5 μg/L
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Characteristics
- Vitamin B2 is a microtiter plate test kit based on a microbiological assay which measures the total Vitamin B2 content in EDTA whole blood.The test kit contains all required reagents, e.g.standard,medium and microtiter plate coated with a specific microorganism, sufficient for 96 determinations including standard curves. An ELISA reader is required for evaluation of the Vitamin B2 content. For use in human and veterinary medicine and in research.
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Vitamin B2 (Riboflavin) ELISA Kit
Reactivity: Various Species Colorimetric Competition ELISA 7.41 ng/mL - 600 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vitamin B2 (Riboflavin) ELISA KitReactivity: Human Colorimetric Sandwich ELISA 10-250 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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Sample Volume
- 100 μL
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Assay Time
- 2 d
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Protocol
- Pretreated EDTA whole blood samples are transferred in the wells of a microtiter plate [PLATE] coated with Lactobacillusrhamnosus.The addition of Vitamin B2 in either standards [STD] or samples gives a Vitamin B2-dependent growth response until Vitamin B2 is consumed. After incubation at 37 °C for 48 hours, the growth of Lactobacillusrhamnosus is measured turbidimetrically at 610 - 630nm (alternatively at 540 - 550nm) in an ELISA-reader and a standard curve is generated from the dilution series.The amount of Vitamin B2 is directly proportional to the turbidity.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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- Vitamin B2 (Vitamin B2 (Riboflavin))
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Alternative Name
- Vitamin B2
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Target Type
- Chemical
Target
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