Myeloperoxidase ELISA Kit

Catalog No. ABIN2648790

This Human Myeloperoxidase ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human Myeloperoxidase.

Quick Overview for Myeloperoxidase ELISA Kit (ABIN2648790)

Target: Myeloperoxidase (MPO)

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum, Plasma

Product Details Myeloperoxidase ELISA Kit

Purpose: Human Myeloperoxidase (MPO) Serum/Plasma ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human myeloperoxidase (MPO) levels in serum and EDTA-plasma samples. The test is useful for detecting elevated levels of myeloperoxidase in serum and plasma samples.

Analytical Method: Quantitative

Sensitivity: .65 ng/ml

Characteristics: Human Myeloperoxidase (MPO) Serum/Plasma ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 2.5 h

Plate: Pre-coated

Protocol: Human Myeloperoxidase (MPO) Serum/Plasma ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human myeloperoxidase in serum and plasma samples. The assay utilizes the two-site "sandwich" technique with selected antibodies that bind to different epitopes of myeloperoxidase Assay standards, controls and diluted patient samples are added directly to wells of a microtiter plate that is coated with antibody to myeloperoxidase. After an incubation period, the plate is washed and horseradish peroxidase (HRP) conjugated human myeloperoxidase antibody is added to each well. After the second incubation period, a "sandwich" of solid-phase monoclonal antibody - human myeloperoxidase - HRP conjugated antibody" is formed. The unbound antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and the absorbances are then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is directly proportional to the amount of human myeloperoxidase in the test sample. A standard curve is generated by plotting the absorbance versus the respective human myeloperoxidase concentration for each standard on a point-to-point or 4-parameter curve fitting. The concentration of human myeloperoxidase in test samples is determined directly from this standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for Myeloperoxidase

Target: Myeloperoxidase (MPO)

Pathways: Chromatin Binding