GM-CSF ELISA Kit

Catalog No. ABIN2648793

This Human GM-CSF ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human GM-CSF.

Quick Overview for GM-CSF ELISA Kit (ABIN2648793)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum, Plasma, Cell Culture Cells

Product Details GM-CSF ELISA Kit

Purpose: Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) concentrations in cell culture supernates, serum, and plasma.

Analytical Method: Quantitative

Sensitivity: 7 pg/ml

Characteristics: Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Protocol: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for GM-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GM-CSF present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for GM-CSF is added to the wells and binds to the combination of capture antibody- GM-CSF in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of GM-CSF present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven GM-CSF standard dilutions and GM-CSF sample concentration determined.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GM-CSF

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin