RFP Western Blot Kit: for RFP Chemiluminescent Western Blotting
DB, WB
Reactivity: Discosoma
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- Target See all RFP products
- RFP (Red Fluorescent Protein (RFP))
- Reactivity
- Discosoma
- Clonality
- Polyclonal
- Application
- Dot Blot (DB), Western Blotting (WB)
- Cross-Reactivity
- Cherry, Tomato (Solanum lycopersicum), Banana, Orange, Plum, Strawberry
- Featured
- Discover our best selling RFP Primary Antibody
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Red Fluorescent Protein (RFP) ELISA Kit
RFP Reactivity: Discosoma Colorimetric Sandwich ELISA Cell Samples, Tissue Lysate
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- Application Notes
- Use Anti-RFP Chemiluminescent Kit for Western Blotting for detection of RFP-tagged recombinant proteins by western blot. his kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.
- Comment
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Detection Kit Type: Chemiluminescent Western Blot Kit
- Restrictions
- For Research Use only
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- Handling Advice
- Wash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!
- Storage
- RT/4 °C
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- Target
- RFP (Red Fluorescent Protein (RFP))
- Alternative Name
- DsRed Fluorescent Protein (RFP Products)
- Background
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Red Fluorescent Protein (RFP) Western Bloting kit allows for the detection of RFP-tagged recombinant proteins present in cell lysates provided by the user. After protein separation by SDS-PAGE and transfer, the membrane is probed with optimized Anti-RFP antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems.
Synonyms: Western Blotting Kit, Chemiluminescent Kit, Peroxidase Kit, Immunoblotting Kit, RFP, rRFP, mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry
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