Interferon gamma ELISA Kit

Catalog No. ABIN2690556

The Mouse Interferon gamma ELISA Kit (ABIN2690556) is a Colorimetric ELISA Kit designed to quantify Mouse Interferon gamma. This ELISA Kit has been cited in 3 publications.

Quick Overview for Interferon gamma ELISA Kit (ABIN2690556)

Target: Interferon gamma (IFNG)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Serum, Plasma, Cell Culture Supernatant

Product Details Interferon gamma ELISA Kit

Minimum Detection Limit: 0.762 pg/mL

Purpose: The Mouse IFN-γ ELISA Kit II is for the quantitative determination of mouse IFN-γ in serum, plasma, and cell culture supernatant.

Analytical Method: Quantitative

Application Details

Application Notes: The BD Biosciences Pharmingen recombinant mouse IFN-γ presents as a 30 kD homodimeric protein, as shown by SDS-PAGE analysis.

Comment:

BD OptEIA™ Mouse IFN-γ ELISA Kit II

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Handling Advice: Interferon-γ (IFN-γ) is a potent multifunctional cytokine which is secreted by activated NK cells and CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells and exerts its biological effects through specific binding to a single class of high affinity receptors. In addition to its antiviral effects, IFN-γ can upregulate a number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils. IFN-γ exerts strong regulatory influences on the proliferation, differentiation, and effector responses of B cell and T cell subsets. These influences can involve IFN-γ's capacity to boost MHC class I and II expression MHC class I and II expression by antigen-presenting cells as well as direct effects on B cells and T cells themselves.

Storage: 4 °C

Storage Comment: 1. Store unopened kit at 2 - 8°C. Do not use kit after expiration date.
2. Before use, bring all reagents to room temperature (18 - 25°C). Immediately after use, return to proper storage conditions.
3. Lyophilized standards are stable until kit expiration date. After reconstitution, use freshly reconstituted standard within 12 hours (stored at 2 - 8°C).

References for Interferon gamma ELISA Kit (ABIN2690556)

Farrar, Schreiber: "The molecular cell biology of interferon-gamma and its receptor." in: Annual review of immunology, Vol. 11, pp. 571-611, (1993) (PubMed).

Gray, Goeddel: "Cloning and expression of murine immune interferon cDNA." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 19, pp. 5842-6, (1983) (PubMed).

Green, Yeh, Overall: "Rapid, quantitative, semiautomated assay for virus-induced and immune human interferons." in: Journal of clinical microbiology, Vol. 12, Issue 3, pp. 433-8, (1981) (PubMed).

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFN-gamma

Background: Interferon-γ (IFN-γ) is a potent multifunctional cytokine which is secreted by activated NK cells and CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells and exerts its biological effects through specific binding to a single class of high affinity receptors. In addition to its antiviral effects, IFN-γ can upregulate a number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils. IFN-γ exerts strong regulatory influences on the proliferation, differentiation, and effector responses of B cell and T cell subsets. These influences can involve IFN-γ's capacity to boost MHC class I and II expression MHC class I and II expression by antigen-presenting cells as well as direct effects on B cells and T cells themselves.

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy