CCL2 ELISA Kit

Catalog No. ABIN2690571

The Human CCL2 ELISA Kit (ABIN2690571) is a Colorimetric ELISA Kit designed to quantify Human CCL2. This ELISA Kit has been cited in 3 publications.

Quick Overview for CCL2 ELISA Kit (ABIN2690571)

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Serum, Plasma, Cell Culture Supernatant

Product Details CCL2 ELISA Kit

Purpose: The Human MCP-1 ELISA Kit is for the quantitative determination of human MCP-1 in serum, plasma, and cell culture supernatant.

Analytical Method: Quantitative

Components: Antibody Coated Wells - 2 plates of 96 breakable wells (12 strips x 8 wells per plate)
Detection Antibody - 30 mL
Standards - 4 vials, lyophilized
Enzyme Concentrate (250x) - 150 μL
Standard/Sample Diluent - 30 mL
ELISA Diluent - 12 mL
Wash Concentrate (20x) - 100 mL
TMB One-Step Substrate Reagent - 30 mL
Stop Solution - 13 mL
Plate Sealers - 4 each

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

BD OptEIA™ Human MCP-1 ELISA Kit

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 1. Store unopened kit at 2 - 8°C. Do not use kit after expiration date.
2. Before use, bring all reagents to room temperature (18 - 25°C). Immediately after use, return to proper storage conditions.
3. Lyophilized standards are stable until kit expiration date. After reconstitution, use freshly reconstituted standard within 12 hours (stored at 2 - 8°C).

References for CCL2 ELISA Kit (ABIN2690571)

Rollins: "Chemokines." in: Blood, Vol. 90, Issue 3, pp. 909-28, (1997) (PubMed).

Rollins, Stier, Ernst, Wong: "The human homolog of the JE gene encodes a monocyte secretory protein." in: Molecular and cellular biology, Vol. 9, Issue 11, pp. 4687-95, (1990) (PubMed).

Capsoni, Minonzio, Ongari, Zanussi: "A new simplified single-filter assay for 'in vitro' evaluation of chemotaxis of 51Cr-labeled polymorphonuclear leukocytes." in: Journal of immunological methods, Vol. 120, Issue 1, pp. 125-31, (1989) (PubMed).

Target Details for CCL2

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Alternative Name: MCP-1

Background: Human monocyte chemoattractant protein-1 (MCP-1), previously known as MCAF (monocyte chemotactic and activating factor), is a member of the CC chemokine family. MCP-1 is produced by a variety of stimulated cell types including monocytes, lymphocytes, endothelial cells and fibroblasts. MCP-1 is a potent chemoattractant for monocytes and it also activates lymphocytes, basophils and NK cells.

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection