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PRSS22 ELISA Kit

PRSS22 Reactivity: Mouse Colorimetric Sandwich ELISA 60-4.000 pg/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN2702874
  • Target See all PRSS22 ELISA Kits
    PRSS22 (Protease, serine, 22 (PRSS22))
    Reactivity
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    60-4.000 pg/mL
    Minimum Detection Limit
    60 pg/mL
    Application
    ELISA
    Purpose
    Mouse Tryptase epsilon ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Sample Type
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair detects mouse BSSP-4. Other species not determined.
    Sensitivity
    60 pg/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Application Notes
    Recommended Dilution for serum and plasma samples2 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all PRSS22 ELISA Kits
    PRSS22 (Protease, serine, 22 (PRSS22))
    Alternative Name
    BSSP 4 (PRSS22 Products)
    Synonyms
    BSSP-4 ELISA Kit, hBSSP-4 ELISA Kit, 4733401N09Rik ELISA Kit, SP001LA ELISA Kit, protease, serine, 22 ELISA Kit, protease, serine 22 ELISA Kit, Prss22 ELISA Kit, PRSS22 ELISA Kit
    Background
    Gene Names: Prss22 Bssp4 Prss26
    Protein names: Brain-specific serine protease 4 (BSSP-4) (EC 3.4.21.-) (Serine protease 22) (Serine protease 26) (Tryptase epsilon)
    Gene ID
    70835
    UniProt
    Q9ER10
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