IL-4 ELISA Kit

Catalog No. ABIN2703234

This Colorimetric ELISA kit is designed for the quantitative measurement of Cat IL-4.

Quick Overview for IL-4 ELISA Kit (ABIN2703234)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivity: Cat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.3-80 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details IL-4 ELISA Kit

Minimum Detection Limit: 0.3 ng/mL

Purpose: Feline IL-4 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Analytical Method: Quantitative

Specificity: This ELISA kit detects feline IL4. Less than 0.2 % cross-reactivity with recombinant human IL-4, recombinant mouse IL-4, recombinant rat IL-4, recombinant cotton rat IL4, recombinant porcine IL-4, and recombinant canine IL4 is observed in this antibody pair.

Sensitivity: 0.3 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: IL-4

Background: Gene Names: IL4
Protein names: Interleukin-4 (IL-4) (B-cell stimulatory factor 1) (BSF-1) (Lymphocyte stimulatory factor 1)

Gene ID: 751514

UniProt: P55030

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation