NOV ELISA Kit

Catalog No. ABIN2703371

Human NOV ELISA Kit Colorimetric assay for quantification of Human NOV in Plasma, Cell Culture Supernatant and Serum

Quick Overview for NOV ELISA Kit (ABIN2703371)

Target: NOV (Nephroblastoma Overexpressed (NOV))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 2 pg/mL-30 ng/mL

Application: ELISA

Sample Type: Plasma, Cell Culture Supernatant, Serum

Product Details NOV ELISA Kit

Minimum Detection Limit: 2 pg/mL

Purpose: Human NOV (CCN3) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Analytical Method: Quantitative

Specificity: This ELISA antibody pair detects human NOV. Other species not determined yet.

Sensitivity: 2 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 - 20 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for NOV

Target: NOV (Nephroblastoma Overexpressed (NOV))

Alternative Name: NOV

Background: Gene Names: NOV CCN3 IGFBP9 NOVH
Protein names: Protein NOV homolog (NovH) (CCN family member 3) (Insulin-like growth factor-binding protein 9) (IBP-9) (IGF-binding protein 9) (IGFBP-9) (Nephroblastoma-overexpressed gene protein homolog)

Gene ID: 4856

UniProt: P48745

Pathways: Smooth Muscle Cell Migration, Growth Factor Binding