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PGLYRP1 ELISA Kit
PGLYRP1
Reactivity: Human
Colorimetric
Sandwich ELISA
6-1.500 pg/mL
Cell Culture Supernatant, Plasma, Serum
Product Details PGLYRP1 ELISA Kit
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Target
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PGLYRP1
(Peptidoglycan Recognition Protein 1 (PGLYRP1))
Reactivity
All reactivities for PGLYRP1 elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
6-1.500 pg/mL
Minimum Detection Limit
6 pg/mL
Application
ELISA
Purpose
Human PGRPs ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
Specificity
This ELISA antibody pair detects human PGRPs, less than 0.05 % cross-reactivity with human PGRP-1 beta and mouse PGRPs is observed.
Sensitivity
6 pg/mL
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Recommended Dilution for serum and plasma samples100 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for PGLYRP1
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Target
See all PGLYRP1 ELISA Kits
PGLYRP1
(Peptidoglycan Recognition Protein 1 (PGLYRP1))
Alternative Name
PGRPs (PGLYRP1 Products )
Synonyms
PGLYRP1 ELISA Kit, PGLYRP ELISA Kit, pgrp ELISA Kit, tag7 ELISA Kit, pgrps ELISA Kit, pglyrp ELISA Kit, pgrp-s ELISA Kit, tnfsf3l ELISA Kit, MGC108330 ELISA Kit, MGC108469 ELISA Kit, PGRP-SC1a ELISA Kit, si:dkey-113g17.4 ELISA Kit, si:ch211-202c21.3 ELISA Kit, PGRP ELISA Kit, PGRP-S ELISA Kit, PGRPS ELISA Kit, TAG7 ELISA Kit, TNFSF3L ELISA Kit, Pglyrp ELISA Kit, Tag7 ELISA Kit, Tasg7 ELISA Kit, Tnfsf3l ELISA Kit, Pgrp ELISA Kit, peptidoglycan recognition protein 1 ELISA Kit, peptidoglycan recognition protein 1 L homeolog ELISA Kit, peptidoglycan recognition protein 5 ELISA Kit, PGLYRP1 ELISA Kit, pglyrp1.L ELISA Kit, pglyrp1 ELISA Kit, pglyrp5 ELISA Kit, Pglyrp1 ELISA Kit
Background
Gene Names: PGLYRP1 PGLYRP PGRP TNFSF3L SBBI68 UNQ639/PRO1269 Protein names: Peptidoglycan recognition protein 1 (Peptidoglycan recognition protein short) (PGRP-S)
Gene ID
8993
UniProt
O75594
Pathways
Activation of Innate immune Response
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