ADIPOQ ELISA Kit

Catalog No. ABIN2747931

The Rat ADIPOQ ELISA Kit (ABIN2747931) is a Colorimetric ELISA Kit designed to quantify Rat ADIPOQ. This ELISA Kit has been cited in 2 publications.

Quick Overview for ADIPOQ ELISA Kit (ABIN2747931)

Target: ADIPOQ (Adiponectin (ADIPOQ))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.8 ng/mL - 200 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum

Product Details ADIPOQ ELISA Kit

Minimum Detection Limit: 0.8 ng/mL

Purpose: Rat Adiponectin (ACRP30) ELISA Kit for Cell Culture Supernatants and Serum samples.

Analytical Method: Quantitative

Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rat 4-1BB, Activin A, CD80, CD48, CTACK, Decorin, Eotaxin-1, EphA5, Erythropoietin, FGF-BP, Flt-3 Ligand, Galectin-1, Galectin-3, Gas 1, GFR alpha-1, gp130, HGF, IL-1 R6, IL-1 ra, IL-2 R alpha, IL-3, IL-7, IL-17F, IL-22, JAM-A, MIP-1 alpha, Neuropilin-1, Neuropilin-2, Nope, Notch-1, Notch-2, P-Cadherin, Prolactin, RANTES, SCF, TIM-1, TIMP-2, TREM-1, TWEAK R.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Suggested Plasma Dilution: 300 fold

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

References for ADIPOQ ELISA Kit (ABIN2747931)

Thakare, Surana: "β-Asarone modulate adipokines and attenuates high fat diet-induced metabolic abnormalities in Wistar rats." in: Pharmacological research, Vol. 103, pp. 227-35, (2016) (PubMed).

Shihabudeen, Roy, James, Thirumurugan: "Chenodeoxycholic acid, an endogenous FXR ligand alters adipokines and reverses insulin resistance." in: Molecular and cellular endocrinology, Vol. 414, pp. 19-28, (2015) (PubMed).

Target Details for ADIPOQ

Target: ADIPOQ (Adiponectin (ADIPOQ))

Alternative Name: Adiponectin

Background:

Gene Symbols: ADIPOQ, ACDC, RCG_36676

Protein Name (Synonyms): 30 kDa adipocyte complement-related protein (Adiponectin, C1Q and collagen domain containing) (Protein Adipoq)

Gene ID: 246253

UniProt: Q8K3R4