Follistatin ELISA Kit

Catalog No. ABIN2748110

Product Details Follistatin ELISA Kit

Target: Follistatin (FST)

Reactivity: Rhesus Monkey

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Rhesus Macaque Follistatin (FST) ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: The antibody pair provided in this kit recognizes Rhesus Macaque Follistatin (FST)

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Assay Procedure:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Follistatin

Target: Follistatin (FST)

Alternative Name: Follistatin (FST Products)

Synonyms: CG12955 ELISA Kit, CG12956 ELISA Kit, CG33466 ELISA Kit, Dmel\\CG33466 ELISA Kit, Fol1 ELISA Kit, dFS ELISA Kit, dFol1 ELISA Kit, dfs ELISA Kit, fs ELISA Kit, FS ELISA Kit, foll ELISA Kit, xfs ELISA Kit, FST ELISA Kit, fst ELISA Kit, AL033346 ELISA Kit, FOL1 ELISA Kit, Fst-288 ELISA Kit, RATFOL1 ELISA Kit, cb446 ELISA Kit, fd07h10 ELISA Kit, fst1 ELISA Kit, wu:fd07h10 ELISA Kit, Follistatin ELISA Kit, follistatin ELISA Kit, follistatin L homeolog ELISA Kit, follistatin a ELISA Kit, Fs ELISA Kit, fst ELISA Kit, FST ELISA Kit, LOC100141662 ELISA Kit, fst.L ELISA Kit, Fst ELISA Kit, fsta ELISA Kit

Background: Follistatin (FST)

Gene ID: 707420

UniProt: F7AVH0

Pathways: Negative Regulation of Hormone Secretion