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PRAS40 ELISA Kit

AKT1S1 Reactivity: Human pThr246 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Catalog No. ABIN2748556
  • Target See all PRAS40 (AKT1S1) ELISA Kits
    PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))
    Binding Specificity
    pThr246
    Reactivity
    • 3
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human Phospho-PRAS40 (T246) ELISA Kit. This assay semi-quantitatively measures phophorylated PRAS40 (Thr246) in lysate samples.
    Sample Type
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificity
    The antibody pair provided in this kit recognizes human PRAS40 phosphorylated at site Threonine-246
    Characteristics
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product AKT1S1 ELISA Kit
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Assay Procedure

    Prepare all reagents and samples as instructed in the manual.
    Add 100 μL of sample or positive control to each well.
    Incubate 2.5 h at RT or O/N at 4 °C.
    Add 100 μL of prepared primary antibody to each well.
    Incubate 1 h at RT.
    Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    Incubate 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target See all PRAS40 (AKT1S1) ELISA Kits
    PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))
    Alternative Name
    PRAS40 (AKT1S1 Products)
    Synonyms
    MGC81452 ELISA Kit, lobe ELISA Kit, pras40 ELISA Kit, fb34a04 ELISA Kit, wu:fb34a04 ELISA Kit, Lobe ELISA Kit, PRAS40 ELISA Kit, 1110012J22Rik ELISA Kit, AI227026 ELISA Kit, Lobel ELISA Kit, AKT1 substrate 1 (proline rich) S homeolog ELISA Kit, AKT1 substrate 1 ELISA Kit, AKT1 substrate 1 (proline rich) ELISA Kit, AKT1 substrate 1 (proline-rich) ELISA Kit, akt1s1.S ELISA Kit, AKT1S1 ELISA Kit, akt1s1 ELISA Kit, Akt1s1 ELISA Kit
    Background
    40 kDa Proline-rich Akt1 Substrate (PRAS40 / AKT1S1 / Lobe) phosphorylated at Threonine-246
    Gene ID
    84335
    UniProt
    Q96B36
    Pathways
    Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Autophagy, BCR Signaling, Warburg Effect
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