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PTGR1 ELISA Kit

PTGR1 Reactivity: Various Species Colorimetric Competition ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Catalog No. ABIN2851335
  • Target See all PTGR1 ELISA Kits
    PTGR1 (Prostaglandin Reductase 1 (PTGR1))
    Reactivity
    Various Species
    Detection Method
    Colorimetric
    Method Type
    Competition ELISA
    Detection Range
    0.625 ng/mL - 40 ng/mL
    Minimum Detection Limit
    0.625 ng/mL
    Application
    ELISA
    Purpose
    The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of this index in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
    Sample Type
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of this index.
    Sensitivity
    0.28 ng/mL
    Components
    • Pre-coated, ready to use 96-well strip plate
    • Standard (freeze dried)
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • TMB
    • Stop Solution
    • Wash Buffer (30X)
    • Plate sealer for 96 wells
    • Instruction manual
    Material not included
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
    Top Product
    Discover our top product PTGR1 ELISA Kit
  • Sample Volume
    50 μL
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards
    2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C
    3. Aspirate and wash 3 times
    4. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
    5. Aspirate and wash 5 times
    6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
    7. Add 50µL Stop Solution. Read at 450 nm immediately.
    Assay Procedure

    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to the index has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled the index and unlabeled the index (Standards or samples) with the pre-coated antibody specific to the index. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of the index in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of the index in the sample.

    Restrictions
    For Research Use only
  • Precaution of Use
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Handling Advice
    The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
    Storage
    4 °C,-20 °C
    Storage Comment
    The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
    Expiry Date
    12 months
  • Target See all PTGR1 ELISA Kits
    PTGR1 (Prostaglandin Reductase 1 (PTGR1))
    Alternative Name
    LTB4 (PTGR1 Products)
    Synonyms
    LTB4DH ELISA Kit, PGR1 ELISA Kit, ZADH3 ELISA Kit, 2510002C21Rik ELISA Kit, Ltb4dh ELISA Kit, Dig1 ELISA Kit, ltb4dh ELISA Kit, wu:fj35e05 ELISA Kit, zgc:101689 ELISA Kit, PTGR1 ELISA Kit, prostaglandin reductase 1 ELISA Kit, PTGR1 ELISA Kit, Ptgr1 ELISA Kit, ptgr1 ELISA Kit
    Background
    Alternative name: LT-B4
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