ADAM8 ELISA Kit

Catalog No. ABIN2859283

Human ADAM8 ELISA Kit Colorimetric assay for quantification of Human ADAM8.

Quick Overview for ADAM8 ELISA Kit (ABIN2859283)

Target: ADAM8 (ADAM Metallopeptidase Domain 8 (ADAM8))

Binding Specificity: AA 1-497

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum

Product Details ADAM8 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ADAM8

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: NSO, M1-P497

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: M1-P497

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Tissue Specificity: Expressed on neutrophils and monocytes.

Plate: Pre-coated

Protocol: human ADAM8 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAM8 has been precoated onto 96-well plates. Standards(NSO, M1-P497) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAM8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADAM8 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human ADAM8 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ADAM8 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 0.22, Standard deviation: 0.013, CV(%): 5.7
• Sample 2: n=16, Mean(ng/ml): 1.47, Standard deviation: 0.090, CV(%): 6.1
• Sample 3: n=16, Mean(ng/ml): 2.34, Standard deviation: 0.185, CV(%): 7.9,
• Sample 1: n=24, Mean(ng/ml): 0.27, Standard deviation: 0.018, CV(%): 6.8
• Sample 2: n=24, Mean(ng/ml): 1.56, Standard deviation: 0.115, CV(%): 7.4
• Sample 3: n=24, Mean(ng/ml): 2.58, Standard deviation: 0.214, CV(%): 8.3

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for ADAM8

Target: ADAM8 (ADAM Metallopeptidase Domain 8 (ADAM8))

Alternative Name: ADAM8

Background:

Protein Function: Possible involvement in extravasation of leukocytes.

Background: A Disintegrin and metalloproteinase domain-containing protein 8 is an enzyme that in humans is encoded by the ADAM8 gene. ADAM8 is localized to chromosome 10q26.3. This gene encodes a member of the ADAM (a disintegrin and metalloproteinase domain) family. Members of the ADAM family, such as ADAM8, are cell surface proteases involved in remodeling of extracellular matrix, cell migration, and processing of membrane-bound signaling molecules. They are characterized by disintegrin and metalloprotease domains that confer adhesive properties and proteolytic activities, respectively. And the protein encoded by this gene may be involved in cell adhesion duringneurodegeneration.

Synonyms: Disintegrin and metalloproteinase domain-containing protein 8,ADAM 8,3.4.24.-,Cell surface antigen MS2,CD156a,ADAM8,MS2,

Full Gene Name: Disintegrin and metalloproteinase domain-containing protein 8

Cellular Localisation: Membrane, Single-pass type I membrane protein.

Gene ID: 101

UniProt: P78325

Pathways: Activation of Innate immune Response, M Phase