ADAM8 ELISA Kit

Catalog No. ABIN6719690

Supplier Product No.: koa0372

This Colorimetric ELISA kit is designed for the quantitative measurement of Human ADAM8.

Quick Overview for ADAM8 ELISA Kit (ABIN6719690)

Target: ADAM8 (ADAM Metallopeptidase Domain 8 (ADAM8))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum

Product Details ADAM8 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Supplier Product No.: koa0372

Supplier: Rockland

Purpose: Human ADAM8 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: M1-P497

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Comment:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human ADAM8 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. It is recommended that each human ADAM8 standard solution and each sample be measured in duplicate.

Assay Precision: Intra-Assay Precision (CV%): 7.5%, 5.6%, 5.4%
Inter-Assay Precision (CV%): 7.5%, 6.6%, 6.8%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for ADAM8

Target: ADAM8 (ADAM Metallopeptidase Domain 8 (ADAM8))

Alternative Name: ADAM8

Background:

Background: A Disintegrin and metalloproteinase domain-containing protein 8 is an enzyme that in humans is encoded by the ADAM8 gene. ADAM8 is localized to chromosome 10q26.3. This gene encodes a member of the ADAM (a disintegrin and metalloproteinase domain) family. Members of the ADAM family, such as ADAM8, are cell surface proteases involved in remodeling of extracellular matrix, cell migration, and processing of membrane-bound signaling molecules. They are characterized by disintegrin and metalloprotease domains that confer adhesive properties and proteolytic activities, respectively. And the protein encoded by this gene may be involved in cell adhesion duringneurodegeneration.

Gene Full Name: ADAM metallopeptidase domain 8

Gene ID: 101

NCBI Accession: NP_001100

UniProt: P78325

Pathways: Activation of Innate immune Response, M Phase