IL-25 ELISA Kit

Catalog No. ABIN2859300

This Human IL-25 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human IL-25. This ELISA Kit has been cited in1 publication.

Quick Overview for IL-25 ELISA Kit (ABIN2859300)

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Binding Specificity: AA 33-177

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details IL-25 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-17E/IL-25

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: Y33-G177

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: Y33-G177

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the IL-17 family.

Tissue Specificity: Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea.

Plate: Pre-coated

Protocol: human IL-17E ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-17E has been precoated onto 96-well plates. Standards(E.coli, Y33-G177) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17E is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-17E amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human IL-17E standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human IL-17E standard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 621, Standard deviation: 24.2, CV(%): 3.9
• Sample 2: n=16, Mean(pg/ml): 1969, Standard deviation: 90.6, CV(%): 4.6
• Sample 3: n=16, Mean(pg/ml): 2795, Standard deviation: 150.9, CV(%): 5.4,
• Sample 1: n=24, Mean(pg/ml): 730, Standard deviation: 30.7, CV(%): 4.2
• Sample 2: n=24, Mean(pg/ml): 2103, Standard deviation: 119.9, CV(%): 5.7
• Sample 3: n=24, Mean(pg/ml): 3065, Standard deviation: 208.4, CV(%): 6.8

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for IL-25 ELISA Kit (ABIN2859300)

Meng, Shen, Shen, Zhou, Shen, Das: "Effect of n-3 and n-6 unsaturated fatty acids on prostate cancer (PC-3) and prostate epithelial (RWPE-1) cells in vitro." in: Lipids in health and disease, Vol. 12, pp. 160, (2014) (PubMed).

Target Details for IL-25

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Alternative Name: IL25

Background:

Protein Function: Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.

Background: Interleukin-25 (IL-25), also known as interleukin-17E (IL-17E), is a protein that in humans is encoded by the IL25 gene. It is a cytokine that belongs to the IL-17 cytokine family and mapped to 14q11.2. IL-25 is secreted by type 2 helper T cells (Th2) and mast cells. It has been found that IL-25 can induce Th2-type cytokine expression and production by non-T, non-B accessory cells that express high levels of major histocompatibility complex class II and low levels of Cd11c. This cytokine also can induce NF-i°B activation, and stimulate the production of IL8. What’s more, IL-25 is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract.

Synonyms: Interleukin-25,IL-25,Interleukin-17E,IL-17E,IL25,IL17E,UNQ3120/PRO10272,

Full Gene Name: Interleukin-25

Cellular Localisation: Secreted.

Gene ID: 64806

UniProt: Q9H293