HMOX1 ELISA Kit

Catalog No. ABIN2859305

This Colorimetric ELISA kit is designed for the quantitative measurement of Human HMOX1. There is 1 publication available.

Quick Overview for HMOX1 ELISA Kit (ABIN2859305)

Target: HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))

Binding Specificity: AA 1-288

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 93.7-6000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details HMOX1 ELISA Kit

Minimum Detection Limit: 93.7 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human HO-1/HMOX1

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: E.coli, M1-M288

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: M1-M288

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Tissue Specificity: Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level). .

Plate: Pre-coated

Protocol: human HO-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for HO-1 has been precoated onto 96-well plates. Standards(E.coli, M1-M288) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HO-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human HO-1 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.7pg/m human HO-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human HO-1 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 527, Standard deviation: 19, CV(%): 3.6
• Sample 2: n=16, Mean(pg/ml): 2085, Standard deviation: 91.74, CV(%): 4.4
• Sample 3: n=16, Mean(pg/ml): 3561, Standard deviation: 185.2, CV(%): 5.2,
• Sample 1: n=24, Mean(pg/ml): 686, Standard deviation: 31.6, CV(%): 4.6
• Sample 2: n=24, Mean(pg/ml): 2664, Standard deviation: 151.8, CV(%): 5.7
• Sample 3: n=24, Mean(pg/ml): 3724, Standard deviation: 242.1, CV(%): 6.5

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for HMOX1 ELISA Kit (ABIN2859305)

Huang, Zhu, Li, Sui, Min: "Effect of Blueberry Anthocyanins Malvidin and Glycosides on the Antioxidant Properties in Endothelial Cells." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 1591803, (2017) (PubMed).

Target Details for HMOX1

Target: HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))

Alternative Name: HMOX1

Background:

Protein Function: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.

Background: HMOX1 (heme oxygenase (decycling) 1) is a human gene that encodes for the enzyme heme oxygenase 1. It is localized to chromosome 22. Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.

Synonyms: Heme oxygenase 1,HO-1,1.14.99.3,HMOX1,HO, HO1,

Full Gene Name: Heme oxygenase 1

Cellular Localisation: Microsome . Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side.

Gene ID: 3162

UniProt: P09601

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interactome