PDGF-BB Homodimer ELISA Kit

Catalog No. ABIN2859308

This Colorimetric ELISA kit is designed for the quantitative measurement of Human PDGF-BB Homodimer. There are 2 publications available.

Quick Overview for PDGF-BB Homodimer ELISA Kit (ABIN2859308)

Target: PDGF-BB Homodimer

Binding Specificity: AA 82-190

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details

Minimum Detection Limit: 31.2 pg/mL

Purpose: Sandwich Human PDGF-BB ELISA Kit to quantitate Human PDGF-BB in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml .

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: S82-T190

Capture antibody and Detection antibody: a monoclonal antibody from mouse, a biotinylated polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 4.4%, 6.6%, 5%
Inter-Assay Precision (CV%): 5.9%, 6.6%, 5%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References

Dai, Cui, Zhang, Cheng, Liu, Yang, Fan, Wang, Lin, Zhang, Li, Mao, Wang, Su, Zhang, Peng, Yang, Hu, Yang, Huang, Xiang, Yu, Zhou, Wei, Deng: "SARI inhibits angiogenesis and tumour growth of human colon cancer through directly targeting ceruloplasmin." in: Nature communications, Vol. 7, pp. 11996, (2018) (PubMed).

Liang, Wang, Zhang, Lu, Wang: "Expression and functional analysis of platelet-derived growth factor in uterine leiomyomata." in: Cancer biology & therapy, Vol. 5, Issue 1, pp. 28-33, (2006) (PubMed).

Target Details

Target: PDGF-BB Homodimer

Alternative Name: PDGF-BB

Background:

Background: Platelet-derived growth factor subunit B is a protein that in humans is encoded by the PDGFB gene. The protein encoded by this gene is a member of the platelet-derived growth factor family. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. This gene is mapped to 22q13.1. Growth factor plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. This gene plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.

Gene Full Name: platelet derived growth factor subunit B

Gene ID: 5155

UniProt: P01127