LBP ELISA Kit

Catalog No. ABIN2859326

This Colorimetric ELISA kit is designed for the quantitative measurement of Human LBP.

Quick Overview for LBP ELISA Kit (ABIN2859326)

Target: LBP (Lipopolysaccharide Binding Protein (LBP))

Binding Specificity: AA 26-481

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details LBP ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human LBP

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: A26-V481

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <50pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: A26-V481

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.

Tissue Specificity: Detected in blood serum (at protein level). .

Plate: Pre-coated

Protocol: human LBP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for LBP has been precoated onto 96-well plates. Standards(NSO, A26-V481) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LBP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human LBP amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human LBP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human LBP standard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 15.3, Standard deviation: 0.66, CV(%): 4.3
• Sample 2: n=16, Mean(ng/ml): 38, Standard deviation: 1.9, CV(%): 5
• Sample 3: n=16, Mean(ng/ml): 85, Standard deviation: 4.76, CV(%): 5.6,
• Sample 1: n=24, Mean(ng/ml): 20, Standard deviation: 1.48, CV(%): 7.4
• Sample 2: n=24, Mean(ng/ml): 42, Standard deviation: 2.77, CV(%): 6.6
• Sample 3: n=24, Mean(ng/ml): 93, Standard deviation: 6.7, CV(%): 7.2

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for LBP

Target: LBP (Lipopolysaccharide Binding Protein (LBP))

Alternative Name: LBP

Background:

Protein Function: Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria (PubMed:7517398, PubMed:24120359). Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide (PubMed:7517398, PubMed:24120359). .

Background: Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. This gene is mapped to 20q11.23. LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide(or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. It is present in the cerebrospinal fluid of patients with pneumococcal meningitis. The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. LBP is made in the liver during the acute phase of infections and is thought to function as a carrier for LPS and to help control LPS-dependent monocyte responses.

Synonyms: Lipopolysaccharide-binding protein,LBP,LBP,

Full Gene Name: Lipopolysaccharide-binding protein

Cellular Localisation: Secreted . Cytoplasmic granule membrane . Membrane-associated in polymorphonuclear Leukocytes (PMN) granules..

Gene ID: 3929

UniProt: P18428

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process