ADAM9 ELISA Kit

Catalog No. ABIN2859343

Human ADAM9 ELISA Kit Colorimetric assay for quantification of Human ADAM9.

Quick Overview for ADAM9 ELISA Kit (ABIN2859343)

Target: ADAM9 (ADAM Metallopeptidase Domain 9 (ADAM9))

Binding Specificity: AA 29-697

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin)

Product Details ADAM9 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ADAM9

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: A29-D697

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: A29-D697

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Contains 1 disintegrin domain.

Tissue Specificity: Widely expressed. Expressed in chondrocytes. Isoform 2 is highly expressed in liver and heart. .

Plate: Pre-coated

Protocol: human ADAM9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAM9 has been precoated onto 96-well plates. Standards(NSO, A29-D697) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAM9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADAM9 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human ADAM9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ADAM9 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 145, Standard deviation: 7.54, CV(%): 5.2
• Sample 2: n=16, Mean(pg/ml): 363, Standard deviation: 20.69, CV(%): 5.7
• Sample 3: n=16, Mean(pg/ml): 594, Standard deviation: 36.23, CV(%): 6.1,
• Sample 1: n=24, Mean(pg/ml): 176, Standard deviation: 11.44, CV(%): 6.5
• Sample 2: n=24, Mean(pg/ml): 380, Standard deviation: 28.12, CV(%): 7.4
• Sample 3: n=24, Mean(pg/ml): 658, Standard deviation: 51.98, CV(%): 7.9

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for ADAM9

Target: ADAM9 (ADAM Metallopeptidase Domain 9 (ADAM9))

Alternative Name: ADAM9

Background:

Protein Function: Probable zinc protease. May mediate cell-cell or cell- matrix interactions. Isoform 2 displays alpha-secretase activity for APP. .

Background: Disintegrin and metalloproteinase domain-containing protein 9, also known as MCMP or MDC9 is an enzyme that in humans is encoded by the ADAM9 gene. This gene encodes a member of the ADAM('a disintegrin and metalloproteinase') family and exhibits a number of characteristics of class III reprolysins. This gene is mapped to 8p11.22. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis.

Synonyms: Disintegrin and metalloproteinase domain-containing protein 9,ADAM 9,3.4.24.-,Cellular disintegrin-related protein,Meltrin-gamma,Metalloprotease/disintegrin/cysteine-rich protein 9,Myeloma cell metalloproteinase,ADAM9,KIAA0021, MCMP, MDC9, MLTNG,

Full Gene Name: Disintegrin and metalloproteinase domain-containing protein 9

Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein.

Gene ID: 8754

UniProt: Q13443

Pathways: Cellular Response to Molecule of Bacterial Origin, SARS-CoV-2 Protein Interactome