APOA1 ELISA Kit

Catalog No. ABIN3044715

Human APOA1 ELISA Kit, Colorimetric assay for quantification of Human APOA1, that has been mentioned in 2+ publications.

Quick Overview for APOA1 ELISA Kit (ABIN3044715)

Target: APOA1 (Apolipoprotein A-I (APOA1))

Binding Specificity: AA 25-267

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.12 ng/mL - 200 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Urine, Serum, Plasma (heparin), Plasma (EDTA)

Product Details APOA1 ELISA Kit

Minimum Detection Limit: 3.12 ng/mL

Purpose: Sandwich Human APOA1 / Apoa I / Apolipoprotein AI ELISA Kit to quantitate Human APOA1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: D25-Q267

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 50 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 4.6%, 7.3%, 5.2%
Inter-Assay Precision (CV%): 6.2%, 7.9%, 6.1%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for APOA1 ELISA Kit (ABIN3044715)

Ding, Luo, Yu, Zhou, Wu: "Vitreous levels of apolipoprotein A1 and retinol binding protein 4 in human rhegmatogenous retinal detachment associated with choroidal detachment." in: Molecular vision, Vol. 24, pp. 252-260, (2018) (PubMed).

Zhao, Li, Zhang, Gao, Pei, Wang, Guo, Yu, Zheng, Wang: "Detection of Serum Protein Biomarkers for the Diagnosis and Staging of Hepatoblastoma." in: International journal of molecular sciences, Vol. 16, Issue 6, pp. 12669-85, (2016) (PubMed).

Target Details for APOA1

Target: APOA1 (Apolipoprotein A-I (APOA1))

Alternative Name: APOA1

Background:

Background: Apolipoprotein A1 is a protein that in humans is encoded by the APOA1 gene. This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.

Gene Full Name: apolipoprotein A1

Gene ID: 335

UniProt: P02647

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism