IgG2a ELISA Kit
Quick Overview for IgG2a ELISA Kit (ABIN3198441)
Target
See all IgG2a productsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 1.95 ng/mL
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Purpose
- This assay employs the quantitative sandwich enzyme immunoassay technique for quantitative detection.
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Analytical Method
- Quantitative
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Specificity
- IgG2a ELISA Kit, Mouse
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Components
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- Plate
- Standard
- Diluent
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Material not included
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Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.
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IgG2a ELISA Kit
Reactivity: Mouse Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
IgG2a ELISA KitReactivity: Guinea Pig Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
IgG2a ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 12.5 ng/mL - 400 ng/mL Plasma, Serum, Urine
IgG2a ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG2a ELISA KitQuick Reactivity: Mouse Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Biological Fluids, Plasma, Serum
IgG2a ELISA KitReactivity: Rat Colorimetric Competition ELISA 246.91 ng/mL - 20000 ng/mL Plasma, Serum
IgG2a ELISA KitReactivity: Rat Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
IgG2a ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
IgG2a ELISA KitReactivity: Guinea Pig Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Sample Volume
- 100 μL
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Assay Time
- 3 - 4 h
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Plate
- Pre-coated
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Restrictions
- For Research Use only
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- protect your eyes
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Storage
- 4 °C
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Storage Comment
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Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit
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- IgG2a
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Target Type
- Antibody
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Background
- Immunoglobulin G (IgG) is a type of antibody created and released by plasma B cells. IgG is the main type of antibody in humoral immunity found in blood and extracellular fluid allowing it to control infection of body tissues.
Target See all IgG2a products
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