BID ELISA Kit

Catalog No. ABIN3199199

The Human BID ELISA Kit (ABIN3199199) is a Colorimetric ELISA Kit designed to quantify Human BID.

Quick Overview for BID ELISA Kit (ABIN3199199)

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.63 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details BID ELISA Kit

Minimum Detection Limit: 15.63 pg/mL

Purpose: This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human BID.

Analytical Method: Quantitative

Specificity: BID ELISA Kit, human

Components:

• Plate
• Standard
• Diluent

Material not included: Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: protect your eyes

Storage: 4 °C

Storage Comment: Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit

Target Details for BID

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Alternative Name: BID

Background: The BH3 interacting domain death agonist (BID), containing only the BH3 domain, is a pro-apoptotic member of the Bcl-2 protein family. BID is important to cell death mediated by Bcl-2 family proteases and thus is the sentinel to protease-mediated death signals. In response to apoptotic signaling, BID interacts with another Bcl-2 family protein, Bax, leading to the insertion of Bax into organelle membranes, primarily the outer mitochondrial membrane.

Gene ID: 637

NCBI Accession: NM_001196

UniProt: P55957

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity