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TERT ELISA Kit

TERT Reactivity: Human Colorimetric Sandwich ELISA 0.312-20 ng/mL Cell Culture Supernatant, Serum, Tissue Homogenate, Urine
Catalog No. ABIN366749
  • Target See all TERT ELISA Kits
    TERT (Telomerase Reverse Transcriptase (TERT))
    Reactivity
    • 7
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.312-20 ng/mL
    Minimum Detection Limit
    0.312 ng/mL
    Application
    ELISA
    Purpose
    For the quantitative determination of human telomerase (TE) concentrations in serum, cell culture supernates, urine, tissue homogenates.
    Sample Type
    Serum, Cell Culture Supernatant, Urine, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of human TE.
    Cross-Reactivity (Details)
    Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.
    Sensitivity
    0.078 ng/mL
    Components
    • Assay plate (12 × 8 coated Microwells)
    • Standard (freeze dried)
    • Biotin-antibody (100 × concentrate)
    • HRP-avidin (100 × concentrate)
    • Biotin-antibody Diluent
    • HRP-avidin Diluent
    • Sample Diluent
    • Wash Buffer (25 × concentrate)
    • TMB Substrate
    • Stop Solution
    • Adhesive Strip (for 96 wells)
    • Instruction manual
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  • Application Notes
    • The supplier is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.
    • Samples to be used within 5 days may be stored at 2-8°C, otherwise samples must be stored at -20°C (≤ 1 month) or -80°C (≤ 2 months) to avoid loss of bioactivity and contamination.
    • Grossly hemolyzed samples are not suitable for use in this assay.
    • If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
    • Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
    • Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts of certain chemicals.
    • Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
    • Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
    • Fresh samples without long time storage are recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.
    Comment

    Detection wavelength: 450 nm

    Information on standard material:
    Depending on the antigen to be detected, standards can be either native or recombinant protein. The recombinant proteins are being expressed in CHO cells in most cases. Please inquire for more information. The formulation of auxiliary material in the standard is considered proprietary information, however it does not contain any poisonous substance. Proclin 300 (1:3000) is used as preservative.

    Information on reagents:
    In most cases the stop solution provided is 1 N H2SO4. The formulation of wash solution is proprietary information. None of the components contain (sodium) azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials. For the sandwich method kits, the sample diluent, antibody diluent, enzyme diluent and standard all contain BSA.

    Information on antibodies:
    The antibodies provided in different kits vary in regards to clonality and host. Some antibodies are affinity purified, some are Protein A

    Sample Volume
    100 μL
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TE bound in the initial step. The color development is stopped and the intensity of the color is measured.
    Assay Precision
    Intra-assay precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess precision.
    Inter-assay precision (precision between assays): Three samples of known concentration were tested in twenty assays to assess precision.
    • Intra-assay: CV% less than 8%
    • Inter-assay: CV% less than 10%
    Restrictions
    For Research Use only
  • Precaution of Use
    The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
    Handling Advice
    • The kit should not be used beyond the expiration date on the kit label.
    • Do not mix or substitute reagents with those from other lots or sources.
    • If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
    • Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
    • This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.
    Storage
    4 °C/-20 °C
    Storage Comment
    For unopened kit: All the reagents should be kept according to the labels on vials.
    Expiry Date
    6 months
  • Pawełczyk, Grancow-Grabka, Trafalska, Szemraj, Żurner, Pawełczyk: "Telomerase level increase is related to n-3 polyunsaturated fatty acid efficacy in first episode schizophrenia: Secondary outcome analysis of the OFFER randomized clinical trial." in: Progress in neuro-psychopharmacology & biological psychiatry, Vol. 83, pp. 142-148, (2019) (PubMed).

  • Target See all TERT ELISA Kits
    TERT (Telomerase Reverse Transcriptase (TERT))
    Alternative Name
    telomerase (TE) (TERT Products)
    Synonyms
    CMM9 ELISA Kit, DKCA2 ELISA Kit, DKCB4 ELISA Kit, EST2 ELISA Kit, PFBMFT1 ELISA Kit, TCS1 ELISA Kit, TP2 ELISA Kit, TRT ELISA Kit, hEST2 ELISA Kit, hTRT ELISA Kit, chTERT ELISA Kit, Tert ELISA Kit, ATTERT ELISA Kit, F5E19.190 ELISA Kit, F5E19_190 ELISA Kit, telomerase reverse transcriptase ELISA Kit, tert ELISA Kit, TR ELISA Kit, est2 ELISA Kit, tcs1 ELISA Kit, telomerase ELISA Kit, tert-A ELISA Kit, tp2 ELISA Kit, trt ELISA Kit, xTERT ELISA Kit, telomerase reverse transcriptase ELISA Kit, Telomerase Reverse Transcriptase ELISA Kit, Tertp ELISA Kit, Telomerase catalytic subunit ELISA Kit, telomerase reverse transcriptase L homeolog ELISA Kit, TERT ELISA Kit, trt-1 ELISA Kit, Tert ELISA Kit, tert ELISA Kit, CMU_003490 ELISA Kit, VDBG_04487 ELISA Kit, GL50803_16225 ELISA Kit, tert.L ELISA Kit
    Pathways
    Telomere Maintenance
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