IL1B ELISA Kit (Interleukin 1, beta)

Details for Product IL1B ELISA Kit No. ABIN411293, Supplier: Log in to see
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL1B
AA 117-269
Kits with alternative reactivity to:
Method Type
Sandwich ELISA
Detection Range
3.9-250 pg/mL
Minimum Detection Limit
3.9 pg/mL
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Supplier Product No.
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'Independent Validation' Badge
Antigen IL1B
Lot Number 1141380914
Method validated ELISA
Positive Control Plasma from LPS stimulated human whole blood; Recombinant human IL-1B with increasing concentrations of canakinumab.
Negative Control Performed following the instructions provided by the manufacturer.

Passed. ABIN411293 detects IL-1B in plasma from human whole blood. In the presence of increasing concentrations of canakinumab, false low IL-1B concentrations were detected, limiting the kit’s practicability.

  • Collect blood from volunteers in hirudin coated tubes (Sarstedt, 04.1944.001).
  • Distribute blood samples on 96 well plates using 140µl per well.
  • Add ultra-pure LPS (Invivogen, tlrl-3pelps) to each well to a concentration of 1µg/ml to primer samples for NLRP3 assays.
  • Incubate plates on a shaker (450rpm) in a humidified incubator for 5.5h at 37°C and 5% CO2.
  • For NLRP3 inflammasome activation, add ATP (Invivogen, tlrl-atpl) to each well to a concentration of 1mM.
  • Incubate plates on a shaker (450rpm) in a humidified incubator for 30min at 37°C and 5% CO2.
  • Add 100µl PBS to each well.
  • Centrifuge plates for 5min at 1200rpm at RT and freeze the supernatant from each well at -80°C.
  • For analysis of canakinumab interference with ABIN411293, dilute recombinant IL-1B (BD, 558457) to 800pg/ml in RPMI1640 and incubate it with increasing doses of canakinumab (0µg/ml, 0.0001µg/ml, 0.001µg/ml, 0.01µg/ml, 0.1µg/ml, 1µg/ml, 10µg/ml, 100µg/ml) for 2h at RT.
  • Perform measurements according to the manufacturer’s protocol.
  • Analyze standards and samples in duplicate. Use assay buffer as blank and subtract the mean blank was subtracted from all raw data reads.
  • Use a five parameter logisitic curve to calculate the standard curve.
Experimental Notes
  • The aim was to analyze the ability of ABIN411293 to detect IL-1B in plasma from stimulated human whole blood and in the presence of the therapeutic IL-1B antibody canakinumab.

  • Analysis of stimulated human whole blood showed the expected results, therefore ABIN411293 is suitable to detect IL-1B from plasma of stimulated human whole blood. Of note, increasing doses of canakinumab seem to interfere with the binding of the ELISAs antibodies leading to detection of false low IL-1B concentrations. Therefore, the ELISA may not be used to analyze serum or plasma from patients receiving therapeutic canakinumab.

Validation Images
ELISA validation image for Interleukin 1, beta (IL1B) ELISA Kit (ABIN411293) A. Human anticoagulated whole blood was stimulated with LPS or LPS and ATP. Subsequen...
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-1 beta
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
Analytical Method Quantitative
Detection Method Colorimetric
Immunogen Expression system for standard: E.coli
Immunogen sequence: A117-S269
Specificity Expression system for standard: E.coli,A117-S269
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <0.15pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Plasmids, Primers & others Plasmids, Primers & others IL1B products on genomics-online (e.g. as negative or positive controls)
Alternative Name IL1B (IL1B ELISA Kit Abstract)

Protein Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. .

Background: Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B(NFKB)-regulated genes. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Synonyms: Interleukin-1 beta,IL-1 beta,Catabolin,IL1B,IL1F2,

Full Gene Name: Interleukin-1 beta

Cellular Localisation: Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
Gene ID 3553
UniProt P01584
Pathways NF-kappaB Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sequence similarities: Belongs to the IL-1 family.

Plate Pre-coated
Protocol human IL-1 beta ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1 beta has been precoated onto 96-well plates. Standards (E.coli,A117-S269) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 beta is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-1 beta amount of sample captured in plate.
Assay Procedure

Aliquot 0.1 mL per well of the 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 16.5pg/mL, 7.8pg/mL, 3.9pg/mL human IL-1 betastandard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human IL-1 betastandard solution and each sample is measured in duplicate.

Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 19, Standard deviation: 1.0, CV(%): 5.3
  • Sample 2: n=16, Mean(pg/ml): 41.2, Standard deviation: 2.6, CV(%): 6.3
  • Sample 3: n=16, Mean(pg/ml): 116, Standard deviation: 6.5, CV(%): 5.6,
  • Sample 1: n=24, Mean(pg/ml): 27.5, Standard deviation: 1.6, CV(%): 5.8
  • Sample 2: n=24, Mean(pg/ml): 61.3, Standard deviation: 4.1, CV(%): 6.7
  • Sample 3: n=24, Mean(pg/ml): 179.1, Standard deviation: 12.7, CV(%): 7.1
Restrictions For Research Use only
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months
Supplier Images
ELISA image for Interleukin 1, beta (IL1B) ELISA Kit (ABIN411293) Human IL-1 beta PicoKine ELISA Kit standard curve
Product cited in: Wang, Xie, Yang, Zhang, Wang, Wu, Shen, Xie: "Sulfated Cyclocarya paliurus polysaccharides markedly attenuates inflammation and oxidative damage in lipopolysaccharide-treated macrophage cells and mice." in: Scientific reports, Vol. 7, pp. 40402, 2017 (PubMed).

Jian, Dai, Hu, Yao, Zheng, Zhu: "ox-LDL increases microRNA-29a transcription through upregulating YY1 and STAT1 in macrophages." in: Cell biology international, Vol. 41, Issue 9, pp. 1001-1011, 2017 (PubMed).

Huang, Liu, Ma, Liao, Lu, Huang, Qin, Liu, Fang: "Human cytomegalovirus triggers the assembly of AIM2 inflammasome in THP-1-derived macrophages." in: Journal of medical virology, 2017 (PubMed).

El-Horany, Abd-Ellatif, Watany, Hafez, Okda: "NLRP3 expression and urinary HSP72 in relation to biomarkers of inflammation and oxidative stress in diabetic nephropathy patients." in: IUBMB life, Vol. 69, Issue 8, pp. 623-630, 2017 (PubMed).

Sun, Zhu, Cai, Qiu: "Hypaphorine Attenuates Lipopolysaccharide-Induced Endothelial Inflammation via Regulation of TLR4 and PPAR-γ Dependent on PI3K/Akt/mTOR Signal Pathway." in: International journal of molecular sciences, Vol. 18, Issue 4, 2017 (PubMed).

Ballerini, Diomede, Petragnani, Cicchitti, Merciaro, Cavalcanti, Trubiani: "Conditioned medium from relapsing-remitting multiple sclerosis patients reduces the expression and release of inflammatory cytokines induced by LPS-gingivalis in THP-1 and MO3.13 cell lines." in: Cytokine, Vol. 96, pp. 261-272, 2017 (PubMed).

Li, Tang, Shuai, Jiang, Liu, Wang, Yao, Nie, Xie: "Mannose Receptor Mediates the Immune Response to Ganoderma atrum Polysaccharides in Macrophages." in: Journal of agricultural and food chemistry, Vol. 65, Issue 2, pp. 348-357, 2016 (PubMed).

Background publications Shi, Yang, Kouadir, Yang, Wang, Zhou, Yin, Zhao: "The NALP3 inflammasome is involved in neurotoxic prion peptide-induced microglial activation." in: Journal of neuroinflammation, Vol. 9, pp. 73, 2012 (PubMed).

Qin, Niu, Wang, Xu, Qiao, Gu: "Astragalus embranaceus extract activates immune response in macrophages via heparanase." in: Molecules (Basel, Switzerland), Vol. 17, Issue 6, pp. 7232-40, 2012 (PubMed).

Li, Zhu, Chen, Hu, Chen, Zhang, Li, Fang: "Molecular characterization and functional analysis of MyD88 in Chinese soft-shelled turtle Trionyx sinensis." in: Fish & shellfish immunology, Vol. 30, Issue 1, pp. 33-8, 2011 (PubMed).

Wang, Wu, Li, Zheng, Liu, Zhou, Yuan, Shang, Yao: "Aspirin-triggered lipoxin A4 attenuates LPS-induced pro-inflammatory responses by inhibiting activation of NF-?B and MAPKs in BV-2 microglial cells." in: Journal of neuroinflammation, Vol. 8, pp. 95, 2011 (PubMed).

Chen, Li, Yeh, Lee, Usami, Chien, Chiu: "Synergistic roles of platelet-derived growth factor-BB and interleukin-1beta in phenotypic modulation of human aortic smooth muscle cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 8, pp. 2665-70, 2006 (PubMed).

Voronov, Shouval, Krelin, Cagnano, Benharroch, Iwakura, Dinarello, Apte: "IL-1 is required for tumor invasiveness and angiogenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 5, pp. 2645-50, 2003 (PubMed).

Baek, Ohgi, Rose, Koo, Glass, Rosenfeld: "Exchange of N-CoR corepressor and Tip60 coactivator complexes links gene expression by NF-kappaB and beta-amyloid precursor protein." in: Cell, Vol. 110, Issue 1, pp. 55-67, 2002 (PubMed).

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