TIMP1 ELISA Kit

Catalog No. ABIN411358

Human TIMP1 ELISA Kit, Colorimetric assay for quantification of Human TIMP1, that has been mentioned in 13+ publications.

Quick Overview for TIMP1 ELISA Kit (ABIN411358)

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Binding Specificity: AA 24-207

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva

Product Details TIMP1 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: Sandwich Human TIMP-1 ELISA Kit to quantitate Human TIMP1 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: C24-A207

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 5 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 6.8%, 7%, 6.8%
Inter-Assay Precision (CV%): 7.6%, 7%, 8.4%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for TIMP1 ELISA Kit (ABIN411358)

Sollazzo, Forte, Polverelli, Romano, Perricone, Rossi, Ottaviani, Luatti, Martinelli, Vianelli, Cavo, Palandri, Catani: "Crucial factors of the inflammatory microenvironment (IL-1β/TNF-α/TIMP-1) promote the maintenance of the malignant hemopoietic clone of myelofibrosis: an in vitro study." in: Oncotarget, Vol. 7, Issue 28, pp. 43974-43988, (2018) (PubMed).

Forte, Salvestrini, Corradi, Rossi, Catani, Lemoli, Cavo, Curti: "The tissue inhibitor of metalloproteinases-1 (TIMP-1) promotes survival and migration of acute myeloid leukemia cells through CD63/PI3K/Akt/p21 signaling." in: Oncotarget, Vol. 8, Issue 2, pp. 2261-2274, (2018) (PubMed).

Liu, Zhong, He, Wei, Zheng, Zhang, Bai, Zhong, Zhong: "Effect of low-dose, long-term roxithromycin on airway inflammation and remodeling of stable noncystic fibrosis bronchiectasis." in: Mediators of inflammation, Vol. 2014, pp. 708608, (2015) (PubMed).

Deng, Ling, Liu, Zhao, Lv: "Decreased expression of matrix metalloproteinase-1 in the maternal umbilical serum, trophoblasts and decidua leads to preeclampsia." in: Experimental and therapeutic medicine, Vol. 9, Issue 3, pp. 992-998, (2015) (PubMed).

Gishto, Farrell, Kothapalli: "Tuning composition and architecture of biomimetic scaffolds for enhanced matrix synthesis by murine cardiomyocytes." in: Journal of biomedical materials research. Part A, Vol. 103, Issue 2, pp. 693-708, (2015) (PubMed).

Stio, Martinesi, Treves, Borgioli: "In vitro response of human peripheral blood mononuclear cells to AISI 316L austenitic stainless steel subjected to nitriding and collagen coating treatments." in: Journal of materials science. Materials in medicine, Vol. 26, Issue 2, pp. 100, (2015) (PubMed).

Simmers, Gishto, Vyavahare, Kothapalli: "Nitric oxide stimulates matrix synthesis and deposition by adult human aortic smooth muscle cells within three-dimensional cocultures." in: Tissue engineering. Part A, Vol. 21, Issue 7-8, pp. 1455-70, (2015) (PubMed).

Cavdar, Ozbal, Celik, Ergur, Guneli, Ural, Camsari, Guner: "The effects of alpha-lipoic acid on MMP-2 and MMP-9 activities in a rat renal ischemia and re-perfusion model." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, Vol. 89, Issue 4, pp. 304-14, (2014) (PubMed).

Hopps, Lo Presti, Montana, Canino, Averna, Caimi: "Study of the correlations among some parameters of the oxidative status, gelatinases, and their inhibitors in a group of subjects with metabolic syndrome." in: Mediators of inflammation, Vol. 2014, pp. 510619, (2014) (PubMed).

Li, Zhao, Ren, Xie, Ma, Rong: "Increased expression of matrix metalloproteinase-9 associated with gastric ulcer recurrence." in: World journal of gastroenterology, Vol. 19, Issue 28, pp. 4590-5, (2014) (PubMed).

Xu, Ling, Zhu, Fan, Zhang: "The effect of 2,3,4',5-tetrahydroxystilbene-2-0-?-D glucoside on neointima formation in a rat artery balloon injury model and its possible mechanisms." in: European journal of pharmacology, Vol. 698, Issue 1-3, pp. 370-8, (2013) (PubMed).

Hopps, Lo Presti, Montana, Noto, Averna, Caimi: "Gelatinases and their tissue inhibitors in a group of subjects with metabolic syndrome." in: Journal of investigative medicine : the official publication of the American Federation for Clinical Research, Vol. 61, Issue 6, pp. 978-83, (2013) (PubMed).

Kim, Lee, Choi, Yoo, Yang: "Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13." in: Rheumatology international, Vol. 32, Issue 10, pp. 3069-75, (2012) (PubMed).

Target Details for TIMP1

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Alternative Name: TIMP1

Background:

Background: The tissue inhibitor of metalloproteinases 1 (TIMP1) is also called erythroid-potentiating activity (EPA). The X-linked gene for human TIMP1 is expressed in some but not all inactive X-containing somatic-cell hybrids, suggesting that this gene is either prone to reactivation or variable in its inactivation. Purified EPA specifically stimulates human and murine cells of the erythroid lineage, unlike murine interleukin-3 (IL-3) which stimulates precursor cells from all haematopoietic lineages. TIMP1 is thought to play a regulatory role in connective tissues by forming inactive complexes with those metalloproteinases that are normally responsible for connective tissue turnover. The human gene encoding TIMP has been mapped to the X chromosome in the region Xp11.1-p11.4. The standard product used in this kit is natural TIMP-1 with the molecular mass of 22KDa.

Gene Full Name: TIMP metallopeptidase inhibitor 1

Gene ID: 7076

UniProt: P01033