VEGF ELISA Kit

Catalog No. ABIN411371

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse VEGF. There are 41 publications available.

Quick Overview for VEGF ELISA Kit (ABIN411371)

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Binding Specificity: AA 27-190

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Product Details VEGF ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: Sandwich Mouse VEGF ELISA Kit to quantitate Mouse Vegfa in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: A27-R190

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 2 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 4.8%, 5.3%, 6.8%
Inter-Assay Precision (CV%): 6.5%, 5.6%, 8.5%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for VEGF ELISA Kit (ABIN411371)

Chen, Qiao, Tang, Chen, Liu, Yang, Wei, Zhang, Cheng, Du, Jiang, Hu, Hua: "Modulation of Salmonella Tumor-Colonization and Intratumoral Anti-angiogenesis by Triptolide and Its Mechanism." in: Theranostics, Vol. 7, Issue 8, pp. 2250-2260, (2018) (PubMed).

Li, Ding, Ling, Wu, Meng, Li, Zhang: "Metformin attenuates motility, contraction, and fibrogenic response of hepatic stellate cells in vivo and in vitro by activating AMP-activated protein kinase." in: World journal of gastroenterology, Vol. 24, Issue 7, pp. 819-832, (2018) (PubMed).

Zou, Wang, Liu, Bai, Wang, Liu, Zhang, Xiao, Wang: "Nucleolin mediated pro-angiogenic role of Hydroxysafflor Yellow A in ischaemic cardiac dysfunction: Post-transcriptional regulation of VEGF-A and MMP-9." in: Journal of cellular and molecular medicine, Vol. 22, Issue 5, pp. 2692-2705, (2018) (PubMed).

Cheng, Chen, Su, Su, Hou: "A novel dressing seeded with embryonic artery CD133+ cells and loaded with the Sirt1 agonist SRT1720 accelerates the healing of diabetic ischemic ulcers." in: Experimental and therapeutic medicine, Vol. 15, Issue 6, pp. 5243-5250, (2018) (PubMed).

Wang, Liu, Chen, Chen: "Physicochemical characterization of the oolong tea polysaccharides with high molecular weight and their synergistic effects in combination with polyphenols on hepatocellular carcinoma." in: Biomedicine & pharmacotherapy, Vol. 90, pp. 160-170, (2017) (PubMed).

Li, Dai, Shen, Gao: "Induced migration of endothelial cells into 3D scaffolds by chemoattractants secreted by pro-inflammatory macrophages in situ." in: Regenerative biomaterials, Vol. 4, Issue 3, pp. 139-148, (2017) (PubMed).

Li, Wang, Wang, Zhang, Wu, Yang, Ji, Li, Wang: "Occludin downregulation in high glucose is regulated by SSTR2 via the VEGF/NRP1/Akt signaling pathway in RF/6A cells." in: Experimental and therapeutic medicine, Vol. 14, Issue 2, pp. 1732-1738, (2017) (PubMed).

Sun, Chen, Zhang, Shen, Wang, Wang, Liu, Liu, Liu, Liu, Jin: "β2-Adrenergic Receptor-Mediated HIF-1α Upregulation Mediates Blood Brain Barrier Damage in Acute Cerebral Ischemia." in: Frontiers in molecular neuroscience, Vol. 10, pp. 257, (2017) (PubMed).

Abdel-Gawad, Hassan, Awwad: "Efficiency of calcium phosphate composite nanoparticles in targeting Ehrlich carcinoma cells transplanted in mice." in: Journal of advanced research, Vol. 7, Issue 1, pp. 143-54, (2016) (PubMed).

Sancakdar, Guven, Uysal, Deveci, Gültürk: "Important of Angiopoietic System in Evaluation of Endothelial Damage in Children with Crimean-Congo Hemorrhagic Fever." in: The Pediatric infectious disease journal, Vol. 34, Issue 8, pp. e200-5, (2015) (PubMed).

Jiang, Han, Li, Yang, Liu: "Carboxymethyl chitosan represses tumor angiogenesis in vitro and in vivo." in: Carbohydrate polymers, Vol. 129, pp. 1-8, (2015) (PubMed).

Jiang, Han, Li, Li, Yang, Liu: "Preparation and anti-tumor metastasis of carboxymethyl chitosan." in: Carbohydrate polymers, Vol. 125, pp. 53-60, (2015) (PubMed).

Das, Jargar, Saha, Yendigeri, Singh: "?-tocopherol supplementation prevents lead acetate and hypoxia-induced hepatic dysfunction." in: Indian journal of pharmacology, Vol. 47, Issue 3, pp. 285-91, (2015) (PubMed).

Fu, Chen, Cheng, Wang: "Hypoxia-inducible factor-1? protects cervical carcinoma cells from apoptosis induced by radiation via modulation of vascular endothelial growth factor and p53 under hypoxia." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 21, pp. 318-25, (2015) (PubMed).

Fan, Zhang, Xu, Ke, Ding, Wang, Liu, Ning, Deng, He: "Serum level of vascular endothelial growth factor decreased in chronic ketamine abusers." in: Drug and alcohol dependence, Vol. 152, pp. 57-61, (2015) (PubMed).

Zhang, Chen, Fan, Wang, Zhu: "Overexpression of VEGF183 promotes murine breast cancer cell proliferation in vitro and induces dilated intratumoral microvessels." in: Tumour biology, Vol. 36, Issue 5, pp. 3871-80, (2015) (PubMed).

Chen, Xu, Li, Du, Chen, Liu, Peng, Wang, Song: "Epigallocatechin-3-gallate attenuates lipopolysaccharide-induced mastitis in rats via suppressing MAPK mediated inflammatory responses and oxidative stress." in: International immunopharmacology, Vol. 26, Issue 1, pp. 147-52, (2015) (PubMed).

Guo, Sun, Xu, Zhao, Peng, Wang: "Human umbilical cord mesenchymal stem cells promote peripheral nerve repair via paracrine mechanisms." in: Neural regeneration research, Vol. 10, Issue 4, pp. 651-8, (2015) (PubMed).

Wang, Zeng, Wang, Liu, Xiao, Xiang: "Evaluating the potential bioactivity of a novel compound ER1626." in: PLoS ONE, Vol. 9, Issue 1, pp. e86509, (2014) (PubMed).

Ge, Ding, Zhang, Li, Li: "Effect of angiogenesis inhibitor SU6668 in combination with 5-Fu on liver metastasis from transplantation tumors of human colorectal cancer in nude mice." in: International journal of clinical and experimental medicine, Vol. 7, Issue 10, pp. 3578-82, (2014) (PubMed).

Target Details for VEGF

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Background:

Background: Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1.The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12. The standard product used in this kit is recombinant mouse VEGF164, a 42KDa dimer.

Gene Full Name: vascular endothelial growth factor A

Gene ID: 22339

UniProt: Q00731