IL-18 ELISA Kit

Catalog No. ABIN411383

Product Details IL-18 ELISA Kit

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Binding Specificity: AA 37-194

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IL-18

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: H37-S194

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <1pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: H37-S194

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: rat IL-18 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-18 has been precoated onto 96-well plates. Standards(E.coli, H37-S194) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-18 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat IL-18 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat IL-18 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 126, Standard deviation: 6, CV(%): 4.7
• Sample 2: n=16, Mean(pg/ml): 335, Standard deviation: 19.43, CV(%): 5.8
• Sample 3: n=16, Mean(pg/ml): 592, Standard deviation: 36.7, CV(%): 6.2,
• Sample 1: n=24, Mean(pg/ml): 147, Standard deviation: 7.94, CV(%): 5.4
• Sample 2: n=24, Mean(pg/ml): 392, Standard deviation: 24.7, CV(%): 6.3
• Sample 3: n=24, Mean(pg/ml): 644, Standard deviation: 46.4, CV(%): 7.2

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for IL-18 ELISA Kit (ABIN411383)

Fu, Wang, Cai, Zhao, Fu: "Theaflavin alleviates inflammatory response and brain injury induced by cerebral hemorrhage via inhibiting the nuclear transcription factor kappa β-related pathway in rats." in: Drug design, development and therapy, Vol. 12, pp. 1609-1619, (2018) (PubMed).

Cao, Fang, Lu, Qian, Ma, He, Pi, Yu, Zhou: "Exposure to nickel oxide nanoparticles induces pulmonary inflammation through NLRP3 inflammasome activation in rats." in: International journal of nanomedicine, Vol. 11, pp. 3331-46, (2017) (PubMed).

Dong, Zhong, Chen, Wu, Zhang, Huang, Yang: "Effect of Acupuncture on Endometrial Angiogenesis and Uterus Dendritic Cells in COH Rats during Peri-Implantation Period." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 3647080, (2017) (PubMed).

Tian, Zhu, Yao, Meng, Wang, Xie, Wang: "NLRP3 participates in the regulation of EMT in bleomycin-induced pulmonary fibrosis." in: Experimental cell research, Vol. 357, Issue 2, pp. 328-334, (2017) (PubMed).

Li, Hu, Wang, Zhang, Zhou, Yang, Li, Xiong, Liu, Li, Wu, Zheng: "Autophagy-dependent generation of Axin2+ cancer stem-like cells promotes hepatocarcinogenesis in liver cirrhosis." in: Oncogene, Vol. 36, Issue 48, pp. 6725-6737, (2017) (PubMed).

Meng, Gong, Fang, Song, Wu, Zhou, Qian: "Effect of interferon-γ on NF-κB and cytokine IL-18 and IL-27 in acute pancreatitis." in: Bosnian journal of basic medical sciences, Vol. 13, Issue 2, pp. 114-8, (2014) (PubMed).

Yang, Shen, Chen, Ge: "Role of interleukin 18 in acute lung inflammation induced by gut ischemia reperfusion." in: World journal of gastroenterology, Vol. 11, Issue 29, pp. 4524-9, (2005) (PubMed).

Target Details for IL-18

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Alternative Name: IL18 (IL18 Products)

Background:

Protein Function: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.

Background: Interleukin(IL)-18, also called Interferon-gamma-inducing factor(IGIF), augments natural killer(NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1(Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3

Synonyms: Interleukin-18,IL-18,Interferon gamma-inducing factor,IFN-gamma-inducing factor,Interleukin-1 gamma,IL-1 gamma,Il18,Igif,

Full Gene Name: Interleukin-18

Cellular Localisation: Secreted.

Gene ID: 29197

UniProt: P97636

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome