Lipocalin 2 ELISA Kit

Catalog No. ABIN411403

Rat Lipocalin 2 ELISA Kit, Colorimetric assay for quantification of Rat Lipocalin 2, that has been mentioned in 12+ publications.

Quick Overview for Lipocalin 2 ELISA Kit (ABIN411403)

Target: Lipocalin 2 (LCN2)

Binding Specificity: AA 21-198

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78 pg/mL - 5000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Urine

Product Details Lipocalin 2 ELISA Kit

Minimum Detection Limit: 78 pg/mL

Purpose: Sandwich Rat Lipocalin-2/NGAL ELISA Kit to quantitate Rat Lcn2 in cell culture supernatants, serum, plasma (heparin) and urine.

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: Q21-N198

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 5%, 6.7%, 5.3%
Inter-Assay Precision (CV%): 5.8%, 7.8%, 5.6%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for Lipocalin 2 ELISA Kit (ABIN411403)

Özlülerden, Toktaş, Aybek, Küçükatay, Şen Türk, Zumrutbas: "The renoprotective effects of mannitol and udenafil in renal ischemia-reperfusion injury model." in: Investigative and clinical urology, Vol. 58, Issue 4, pp. 289-295, (2018) (PubMed).

Erkılıç, Kesimci, Alaybeyoğlu, Kılınç, Tural, Yazgan, Gümüş, Sepici Dinçel, Dumlu, Kanbak: "Does remifentanil attenuate renal ischemia-reperfusion injury better than dexmedetomidine in rat kidney?" in: Drug design, development and therapy, Vol. 11, pp. 677-683, (2017) (PubMed).

Wang, Liu, He, Lv, Du: "Esculin improves dyslipidemia, inflammation and renal damage in streptozotocin-induced diabetic rats." in: BMC complementary and alternative medicine, Vol. 15, pp. 402, (2016) (PubMed).

Ozbilgin, Ozkardesler, Akan, Boztas, Ozbilgin, Ergur, Derici, Guneli, Meseri: "Renal Ischemia/Reperfusion Injury in Diabetic Rats: The Role of Local Ischemic Preconditioning." in: BioMed research international, Vol. 2016, pp. 8580475, (2016) (PubMed).

Li, Jia, Ji, Gong, Wang, Zhang, Wang, Zang: "The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats." in: International journal of molecular medicine, Vol. 38, Issue 3, pp. 767-75, (2016) (PubMed).

Guo, Fang, You, Jin, Wang, Hu, Han: "Effects of hydrogen-rich saline on early acute kidney injury in severely burned rats by suppressing oxidative stress induced apoptosis and inflammation." in: Journal of translational medicine, Vol. 13, pp. 183, (2015) (PubMed).

Guo, Zhou, Huang, You, Fang, Wu, Wang, Han: "Astaxanthin attenuates early acute kidney injury following severe burns in rats by ameliorating oxidative stress and mitochondrial-related apoptosis." in: Marine drugs, Vol. 13, Issue 4, pp. 2105-23, (2015) (PubMed).

Liu, Dong, Sun, Liu: "A novel fluid resuscitation protocol: provide more protection on acute kidney injury during septic shock in rats." in: International journal of clinical and experimental medicine, Vol. 7, Issue 4, pp. 919-26, (2014) (PubMed).

Koca, Olguner, Ergür, Altekin, Ta?dö?en, Duru, Girgin, Gündüz, Cilaker M?c?l?, Güzelda?, Akku?: "The effects of dexmedetomidine on secondary acute lung and kidney injuries in the rat model of intra-abdominal sepsis." in: TheScientificWorldJournal, Vol. 2013, pp. 292687, (2013) (PubMed).

Olguner, Koca, Altekin, Ergür, Duru, Girgin, Ta?dö?en, Gündüz, Güzelda?, Akku?, Micili: "Ischemic preconditioning attenuates lipid peroxidation and apoptosis in the cecal ligation and puncture model of sepsis." in: Experimental and therapeutic medicine, Vol. 5, Issue 6, pp. 1581-1588, (2013) (PubMed).

Sun, Meng, Jiang, Liu, Lei, Su, Duan, Wu, Xia, Xia: "Protective effect of ginsenoside Rb1 against intestinal ischemia-reperfusion induced acute renal injury in mice." in: PLoS ONE, Vol. 8, Issue 12, pp. e80859, (2013) (PubMed).

Hur, Garip, Camyar, Ilgun, Ozisik, Tuna, Olukman, Narli Ozdemir, Yildirim Sozmen, Sen, Akcicek, Duman: "The effects of vitamin d on gentamicin-induced acute kidney injury in experimental rat model." in: International journal of endocrinology, Vol. 2013, pp. 313528, (2013) (PubMed).

Target Details for Lipocalin 2

Target: Lipocalin 2 (LCN2)

Alternative Name: LCN2

Background:

Background: Lipocalin-2 (LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. The standard product used in this kit is recombinant rat NGAL, consisting of 177 amino acids with the molecular mass of 21 kDa.

Gene Full Name: lipocalin 2

Gene ID: 170496

UniProt: P30152

Pathways: Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis