CTGF ELISA Kit

Catalog No. ABIN415039

Human CTGF ELISA Kit, Colorimetric assay for quantification of Human CTGF, that has been mentioned in 21+ publications.

Quick Overview for CTGF ELISA Kit (ABIN415039)

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78 ng/mL - 50 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Product Details CTGF ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Purpose: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CTGF in human serum, plasma, tissue homogenates.

We offer validation data (WB) for the kit components. So you can be sure to order a reliable ELISA kit product composed of high quality reagents.

Analytical Method: Quantitative

Specificity:

This assay has high sensitivity and excellent specificity for detection of Connective Tissue Growth Factor (CTGF).
No significant cross-reactivity or interference between Connective Tissue Growth Factor (CTGF) and analogues was observed.

Cross-Reactivity (Details): No significant cross-reactivity or interference between Connective Tissue Growth Factor (CTGF) and analogues was observed.

Sensitivity: < 0.32 ng/mL

Components:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• TMB Substrate
• Stop Solution
• Wash Buffer (30X)
• Instruction manual

Material not included:

• Microplate reader with 450 nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Application Details

Application Notes:

• Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
• The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
• Kits from different batches may be a little different in detection range, sensitivity and color developing time.
• Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
• Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
• There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
• Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
• Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
• Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
• Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.

Comment:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Assay Precision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Connective Tissue Growth Factor (CTGF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Connective Tissue Growth Factor (CTGF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Precaution of Use: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Handling Advice: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Storage: 4 °C

Storage Comment:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Expiry Date: 6 months

References for CTGF ELISA Kit (ABIN415039)

Shieh, Tsai, Chi, Wu: "TGFβ mediates collagen production in human CRSsNP nasal mucosa-derived fibroblasts through Smad2/3-dependent pathway and CTGF induction and secretion." in: Journal of cellular physiology, Vol. 234, Issue 7, pp. 10489-10499, (2020) (PubMed).

Santos-Faria, Gavina, Rodrigues, Coelho, da Costa Martins, Leite-Moreira, Falcão-Pires: "MicroRNAs and ventricular remodeling in aortic stenosis." in: Revista portuguesa de cardiologia : orgão oficial da Sociedade Portuguesa de Cardiologia = Portuguese journal of cardiology : an official journal of the Portuguese Society of Cardiology, Vol. 39, Issue 7, pp. 377-387, (2020) (PubMed).

Rubiś, Wiśniowska-Smiałek, Wypasek, Rudnicka-Sosin, Hlawaty, Leśniak-Sobelga, Kostkiewicz, Podolec et al.: "12-month patterns of serum markers of collagen synthesis, transforming growth factor and connective tissue growth factor are similar in new-onset and chronic dilated cardiomyopathy in patients both ..." in: Cytokine, Vol. 96, pp. 217-227, (2018) (PubMed).

Rubiś, Wiśniowska-Śmiałek, Dziewięcka, Rudnicka-Sosin, Kozanecki, Podolec: "Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events." in: Advances in medical sciences, Vol. 63, Issue 1, pp. 160-166, (2018) (PubMed).

Wu, Su, Wu, Hwang, Lin: "Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with Preserved Ejection Fraction Patients." in: Journal of clinical medicine, Vol. 7, Issue 11, (2018) (PubMed).

Zhang, Qi, Chen, Shi, Bai, Huang, Yu, Jiang, Zhao, Li: "The relationship between anti-vascular endothelial growth factor and fibrosis in proliferative retinopathy: clinical and laboratory evidence." in: The British journal of ophthalmology, Vol. 100, Issue 10, pp. 1443-50, (2016) (PubMed).

Hamed, El-Saied, Saad, Yousef, Mohamed, Sabry: "Molecular mechanisms underlying fibrosis and elastin destruction in childhood interstitial lung diseases." in: Pathophysiology : the official journal of the International Society for Pathophysiology, Vol. 23, Issue 4, pp. 275-283, (2016) (PubMed).

Rubiś, Wiśniowska-Śmialek, Wypasek, Biernacka-Fijalkowska, Rudnicka-Sosin, Dziewiecka, Faltyn, Khachatryan, Karabinowska, Kozanecki, Tomkiewicz-Pająk, Podolec: "Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 65, Issue 12, pp. 941-949, (2016) (PubMed).

Rubiś, Wiśniowska-Śmiałek, Biernacka-Fijałkowska, Rudnicka-Sosin, Wypasek, Kozanecki, Dziewięcka, Faltyn, Karabinowska, Khachatryan, Hlawaty, Leśniak-Sobelga, Kostkiewicz, Płazak, Podolec: "Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR." in: Heart and vessels, Vol. 32, Issue 6, pp. 714-725, (2016) (PubMed).

Westbury, Haviland, Davies, Gothard, Abdi, Sydenham, Bowen, Stratton, Short, Yarnold: "Cytokine levels as biomarkers of radiation fibrosis in patients treated with breast radiotherapy." in: Radiation oncology (London, England), Vol. 9, pp. 103, (2015) (PubMed).

Rosin, Sopel, Falkenham, Lee, Légaré: "Disruption of collagen homeostasis can reverse established age-related myocardial fibrosis." in: The American journal of pathology, Vol. 185, Issue 3, pp. 631-42, (2015) (PubMed).

Wu, Wang, Lee, Chang, Su, Yeh, Su, Chen, Chiang, Hwang, Lin, Tsai: "Connective tissue growth factor and cardiac diastolic dysfunction: human data from the Taiwan diastolic heart failure registry and molecular basis by cellular and animal models." in: European journal of heart failure, Vol. 16, Issue 2, pp. 163-72, (2014) (PubMed).

Shi, Chang, Yang, Zhang, Yu, Wu: "Activation of JNK signaling mediates connective tissue growth factor expression and scar formation in corneal wound healing." in: PLoS ONE, Vol. 7, Issue 2, pp. e32128, (2012) (PubMed).

El Mesallamy, Ahmed, Bassyouni, Ahmed: "Clinical significance of inflammatory and fibrogenic cytokines in diabetic nephropathy." in: Clinical biochemistry, Vol. 45, Issue 9, pp. 646-50, (2012) (PubMed).

Colak, Senates, Coskunpinar, Oltulu, Zemheri, Ozturk, Doganay, Mesci, Yilmaz, Enc, Kiziltas, Ulasoglu, Tuncer: "Concentrations of connective tissue growth factor in patients with nonalcoholic fatty liver disease: association with liver fibrosis." in: Disease markers, Vol. 33, Issue 2, pp. 77-83, (2012) (PubMed).

Piao, Brigstock, Zhu, Zhang, Gao: "Clinical significance of connective tissue growth factor in hepatitis B virus-induced hepatic fibrosis." in: World journal of gastroenterology : WJG, Vol. 18, Issue 18, pp. 2280-6, (2012) (PubMed).

Pantou, Manginas, Alivizatos, Degiannis: "Connective tissue growth factor (CTGF/CCN2): a protagonist in cardiac allograft vasculopathy development?" in: The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation, Vol. 31, Issue 8, pp. 881-7, (2012) (PubMed).

Zhou, Zhang, Zhang, Zhu: "Promotion of bone formation by naringin in a titanium particle-induced diabetic murine calvarial osteolysis model." in: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, Vol. 28, Issue 4, pp. 451-6, (2010) (PubMed).

Gao, Brigstock: "Connective tissue growth factor hammerhead ribozyme attenuates human hepatic stellate cell function." in: World journal of gastroenterology : WJG, Vol. 15, Issue 30, pp. 3807-13, (2009) (PubMed).

Maruyama, Fujita, Taniguchi: "Sequence of a cDNA coding for human IRF-1." in: Nucleic acids research, Vol. 17, Issue 8, pp. 3292, (1989) (PubMed).

Target Details for CTGF

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Alternative Name: CTGF

Background: CCN2, HCS24, IGFBP8, NOV2, Insulin-Like Growth Factor-Binding Protein 8, Hypertrophic Chondrocyte-Specific Protein 24, CCN Family Member 2

UniProt: P29279

Pathways: Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Growth Factor Binding