HAVCR1 ELISA Kit

Catalog No. ABIN416253

The Rat HAVCR1 ELISA Kit (ABIN416253) is a Colorimetric ELISA Kit designed to quantify Rat HAVCR1. This ELISA Kit has been cited in 13 publications.

Quick Overview for HAVCR1 ELISA Kit (ABIN416253)

Target: HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Tissue Homogenate, Urine

Product Details HAVCR1 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Purpose: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Kim1 in Urine,Tissue Homogenate,Cell Culture Supernatant,Biological Fluids

Analytical Method: Quantitative

Specificity:

This assay has high sensitivity and excellent specificity for detection of Kidney Injury Molecule 1 (Kim1).
No significant cross-reactivity or interference between Kidney Injury Molecule 1 (Kim1) and analogues was observed.

Cross-Reactivity (Details): No significant cross-reactivity or interference between Kidney Injury Molecule 1 (Kim1) and analogues was observed.

Sensitivity: < 24.5 pg/mL

Components:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• TMB Substrate
• Stop Solution
• Wash Buffer (30X)
• Instruction manual

Material not included:

• Microplate reader with 450 nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Application Details

Application Notes:

• Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
• The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
• Kits from different batches may be a little different in detection range, sensitivity and color developing time.
• Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
• Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
• There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
• Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
• Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
• Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
• Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.

Comment:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Assay Precision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kidney Injury Molecule 1 (Kim1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kidney Injury Molecule 1 (Kim1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Precaution of Use: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Handling Advice: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Storage: 4 °C

Storage Comment:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Expiry Date: 6 months

References for HAVCR1 ELISA Kit (ABIN416253)

Yan, Zang, Gong, Ren, Wang: "MiR-214-3p exacerbates kidney damages and inflammation induced by hyperlipidemic pancreatitis complicated with acute renal injury." in: Life sciences, Vol. 241, pp. 117118, (2020) (PubMed).

Tekin, Beytur, Cakir, Taslıdere, Erden, Tekin, Sandal: "Protective effect of saxagliptin against renal ischaemia reperfusion injury in rats." in: Archives of physiology and biochemistry, pp. 1-11, (2020) (PubMed).

Granados-Pineda, Uribe-Uribe, García-López, Ramos-Godinez, Rivero-Cruz, Pérez-Rojas: "Effect of Pinocembrin Isolated from Mexican Brown Propolis on Diabetic Nephropathy." in: Molecules (Basel, Switzerland), Vol. 23, Issue 4, (2019) (PubMed).

Washino, Hosohata, Jin, Takai, Miyagawa: "Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats." in: Clinical and experimental pharmacology & physiology, Vol. 45, Issue 3, pp. 261-268, (2018) (PubMed).

Al Zaabi, Al Salam, Al Suleimani, Manoj, Nemmar, Ali: "Gum Acacia Improves Renal Function and Ameliorates Systemic Inflammation, Oxidative and Nitrosative Stress in Streptozotocin-Induced Diabetes in Rats with Adenine-Induced Chronic Kidney Disease." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 45, Issue 6, pp. 2293-2304, (2018) (PubMed).

Long, Yang, Yang, Li, Wang: "Attenuation of renal ischemia/reperfusion injury by oleanolic acid preconditioning via its antioxidant, anti‑inflammatory, and anti‑apoptotic activities." in: Molecular medicine reports, Vol. 13, Issue 6, pp. 4697-704, (2016) (PubMed).

Du, Wu, Qing, Wang, Liang, Yu, Tang: "Systemic and flap inflammatory response associates with thrombosis in flap venous crisis." in: Inflammation, Vol. 38, Issue 1, pp. 298-304, (2015) (PubMed).

Wang, Yan, Wu, Ji, Wang, Zang: "Rosiglitazone attenuates renal injury caused by hyperlipidemic pancreatitis." in: International journal of clinical and experimental pathology, Vol. 8, Issue 5, pp. 4332-43, (2015) (PubMed).

Ucibior, Go??biowska, Adamczyk, Nied?wiecka, Fornal: "The renal effects of vanadate exposure: potential biomarkers and oxidative stress as a mechanism of functional renal disorders--preliminary studies." in: BioMed research international, Vol. 2014, pp. 740105, (2014) (PubMed).

Hosohata, Ando, Takeshita, Misu, Takamura, Kaneko, Fujimura: "Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty rats." in: Diabetes & vascular disease research, Vol. 11, Issue 4, pp. 243-250, (2014) (PubMed).

Ragab, Abdallah, El-Abhar: "Cilostazol renoprotective effect: modulation of PPAR-?, NGAL, KIM-1 and IL-18 underlies its novel effect in a model of ischemia-reperfusion." in: PLoS ONE, Vol. 9, Issue 5, pp. e95313, (2014) (PubMed).

El Morsy, Ahmed, Ahmed: "Attenuation of renal ischemia/reperfusion injury by açaí extract preconditioning in a rat model." in: Life sciences, (2014) (PubMed).

Šebeková, Dušinská, Simon Klenovics, Kollárová, Boor, Kebis, Staruchová, Vlková, Celec, Hodosy, Ba?iak, Tušková, Be?o, Tulinská, Príbojová, Bilani?ová, Pojana, Marcomini, Volkovová: "Comprehensive assessment of nephrotoxicity of intravenously administered sodium-oleate-coated ultra-small superparamagnetic iron oxide (USPIO) and titanium dioxide (TiO2) nanoparticles in rats." in: Nanotoxicology, Vol. 8, Issue 2, pp. 142-57, (2013) (PubMed).

Target Details for HAVCR1

Target: HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))

Alternative Name: Kim1

Target Type: Virus

Background: HAVCR1, TIM1, TIMD1, HAVCR, Hepatitis A Virus Cellular Receptor 1, T Cell Immunoglobulin And Mucin Domain-Containing Protein 1, T-cell immunoglobulin mucin receptor 1

UniProt: O54947