Anti-Aprotinin Antibody (IgG) ELISA Kit

Catalog No. ABIN454011

The Anti-Aprotinin Antibody (IgG) ELISA Kit (ABIN454011) is a Colorimetric ELISA Kit designed to quantify Anti-Aprotinin Antibody (IgG).

Quick Overview for Anti-Aprotinin Antibody (IgG) ELISA Kit (ABIN454011)

Target: Anti-Aprotinin Antibody (IgG)

Detection Method: Colorimetric

Method Type: Competition ELISA

Application: ELISA

Product Details

Analytical Method: Quantitative

Application Details

Plate: Pre-coated

Protocol: Aprotinin has been pre-coated onto a microtiter plate. During incubation the anti-aprotinin antibodies are immobilized on the plate. For detection of bound antibodies an enzyme-linked anti-human-IgG antibody conjugate is added. After washing off any unbound conjugate, a substrate solution is added. The color developing correlates to the amount of bound antibody conjugate. Absorption at 450 nm is proportional to the concentration and/or avidity of anti-aprotinin antibodies.

Assay Procedure:

Incubation of samples/standards/controls: 100 µL 60 min, room temperature.
Wash Incubation of HRP-labelled anti-human IgG: 100 µL 30 min, room temperature.
Wash Substrate incubation: 100 µL/well 15 min, room temperature.
Add stop solution: 100 µL/well.
Read at 450 nm

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details

Target: Anti-Aprotinin Antibody (IgG)

Target Type: Antibody

Background: Aprotinin is used in operations to reduce intra-operative and postoperative bleeding tendency. Aprotinin as an allogeneic protein has antigenic properties. It has been shown that formation of IgG antibodies to aprotinin takes place in about 50% of all treated patients. During aprotinin re-exposure allergic reactions may occur due to these preformed anti-aprotinin antibodies. Letal anaphylactic shocks have been described in literature. This enzyme immunoassay allows the detection of IgG antibodies against aprotinin in human serum. Patients showing positive test results should be excluded from further aprotinin exposure to reduce the risk of allergic reactions. Therefore, this test system is a valuable contribution to increase the safety of aprotinin applications.