Albumin ELISA Kit

Catalog No. ABIN454013

Product Details Albumin ELISA Kit

Target: Albumin (ALB)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Competition ELISA

Minimum Detection Limit: 0.1 µg/mL

Application: ELISA

Purpose: This ELISA allows quantification of albumin in mouse urine, so that an estimation of kidney function is possible.

Sample Type: Urine

Analytical Method: Quantitative

Characteristics: Determination of albumin is carried out as direct competitive ELISA.

Application Details

Plate: Pre-coated

Protocol: Mouse albumin has been pre-coated onto a microtiter plate. During incubation binding of an enzyme-linked anti-albumin(mouse)-antibody is inhibited by albumin from samples/ standards. After washing away any unbound antibody a substrate solution is added. Developing colour correlated with the amount of antibody conjugate. Absorption at 450 nm is reverse proportional to albumin concentration.

Assay Procedure:

Incubation of samples/standards with antibody conjugate: 50 µL + 50 µL/well 90 min with shaking,120 min without shaking.
Wash Substrate incubation: 100 µL/well 10 min, room temperature.
Add stop solution 100 µL/well.
Read at 450 nm

Assay Precision: Intraassay precision (CV):(sample dilution 1:100, n=12) at 3.1 µg/ml: 14.3%at 30.4 µg/ml: 6.1% at 223 µg/ml: 5.8%

Restrictions: For Research Use only

Handling

Storage: 4 °C

References for Albumin ELISA Kit (ABIN454013)

Liebisch, Bondeva, Franke, Hause, Wolf: "Growth arrest specific 2-like protein 1 expression is upregulated in podocytes through advanced glycation end-products." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, (2016) (PubMed).

Schordan, Grisk, Schordan, Miehe, Rumpel, Endlich, Giebel, Endlich: "OPN deficiency results in severe glomerulosclerosis in uninephrectomized mice." in: American journal of physiology. Renal physiology, (2013) (PubMed).

Lee, Auger, Park, Kang, Oak, Kim, Schini-Kerth: "An Ethanolic Extract of Lindera obtusiloba Stems, YJP-14, Improves Endothelial Dysfunction, Metabolic Parameters and Physical Performance in Diabetic db/db Mice." in: PLoS ONE, Vol. 8, Issue 6, pp. e65227, (2013) (PubMed).

Hyvoenen, Saurus, Wasik, Heikkilae, Havana, Trokovic, Saleem, Holthoefer, Lehtonen: "Lipid phosphatase SHIP2 downregulates insulin signalling in podocytes." in: Molecular and cellular endocrinology, Vol. 328, Issue 1-2, pp. 70-9, (2010) (PubMed).

Heikkilae, Juhila, Lassila, Messing, Peraelae, Lehtonen, Lehtonen, Sjef Verbeek, Holthofer: "beta-Catenin mediates adriamycin-induced albuminuria and podocyte injury in adult mouse kidneys." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 25, Issue 8, pp. 2437-46, (2010) (PubMed).

Target Details for Albumin

Target: Albumin (ALB)

Alternative Name: Albumin (ALB Products)

Synonyms: PRO0883 ELISA Kit, PRO0903 ELISA Kit, PRO1341 ELISA Kit, ALB ELISA Kit, CSA ELISA Kit, Alb-1 ELISA Kit, Alb1 ELISA Kit, Albza ELISA Kit, LOC100136344 ELISA Kit, alb-a ELISA Kit, alb-b ELISA Kit, albumin ELISA Kit, serum albumin 1 ELISA Kit, albumin S homeolog ELISA Kit, ALB ELISA Kit, Alb ELISA Kit, LOC100136344 ELISA Kit, alb.S ELISA Kit

Background: When blood is filtered in glomeruli of kidneys, highly molecular substances are held back. Therefore, urine is nearly protein free. In case of kidney damages, small proteins like albumin appear in urine. Albumin therefore serves as a marker in monitoring kidney damages. In examining nephrotoxic substances and therapeutic strategies regarding kidney damages, animal models are frequently used.

Pathways: Lipid Metabolism