Anti-Alpha2 Adrenergic Receptor Antibodies (IgG) ELISA Kit

Catalog No. ABIN454021

Human Anti-Alpha2 Adrenergic Receptor Antibodies (IgG) ELISA Kit, Colorimetric assay for quantification of Human Anti-Alpha2 Adrenergic Receptor Antibodies (IgG).

Quick Overview for Anti-Alpha2 Adrenergic Receptor Antibodies (IgG) ELISA Kit (ABIN454021)

Target: Anti-Alpha2 Adrenergic Receptor Antibodies (IgG)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Application: ELISA

Product Details

Purpose: The anti-alpha2-adrenergic-receptor-antibody-EIA is an antibody screening test. The alpha2-adrenergic-receptor has been pre-coated onto a microtiter plate. During the first incubation the anti-alpha2-adrenergic-receptor-antibodies of the samples are immobilised on the plate. The auto-antibodies are detected with a POD labeled anti-human IgG antibody. In the following enzymatic substrate reaction the intensity of the colour correlates with the concentration and/ or avidity of anti-alpha2-adrenergic-receptor-antibodies.

Analytical Method: Quantitative

Application Details

Plate: Pre-coated

Assay Procedure:

Incubation of samples/ standards/ controls: 100 µL 120 min, 4°C
Wash Incubation of detection antibody: 100 µL 1 hr, room temperature
Wash Substrate incubation: 100 µL/well 20 min, room temperature
Add Stopp solution: 100 µL/well
Read at 450nm

Assay Precision: Intraassay-Precision:9,12% Interassay-Precision:13.44%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details

Target: Anti-Alpha2 Adrenergic Receptor Antibodies (IgG)

Target Type: Antibody

Background: The Alpha-2 adrenergic receptor is a G protein-coupled receptor. ß receptors have the subtypes ß1, ß2 and ß3. Agonist binding causes a rise in the intracellular concentration of the second messenger cAMP. The anti-alpha2-adrenergic-receptor-antibody-EIA is designed for the determination of Antibodies (IgG) against the Alpha2-adrenergic-receptor in serum and plasma.